@article{81143c7e84254d4f9b7ce6bc9f8bdbdf,
title = "The plant pathogen enzyme AldC is a long-chain aliphatic aldehyde dehydrogenase",
abstract = "Aldehyde dehydrogenases are versatile enzymes that serve a range of biochemical functions. Although traditionally considered metabolic housekeeping enzymes because of their ability to detoxify reactive aldehydes, like those generated from lipid peroxidation damage, the contributions of these enzymes to other biological processes are widespread. For example, the plant pathogen Pseudomonas syringae strain PtoDC3000 uses an indole-3-acetaldehyde dehydrogenase to synthesize the phytohormone indole-3-acetic acid to elude host responses. Here we investigate the biochemical function of AldC from PtoDC3000. Analysis of the substrate profile of AldC suggests that this enzyme functions as a long-chain aliphatic aldehyde dehydrogenase. The 2.5 {\AA} resolution X-ray crystal of the AldC C291A mutant in a dead-end complex with octanal and NAD1 reveals an apolar binding site primed for aliphatic aldehyde substrate recognition. Functional characterization of site-directed mutants targeting the substrate- and NAD(H)-binding sites identifies key residues in the active site for ligand interactions, including those in the “aromatic box” that define the aldehyde-binding site. Overall, this study provides molecular insight for understanding the evolution of the prokaryotic aldehyde dehydrogenase superfamily and their diversity of function.",
author = "Lee, {Soon Goo} and Kate Harline and Orchid Abar and Akadri, {Sakirat O.} and Bastian, {Alexander G.} and Chen, {Hui Yuan S.} and Michael Duan and Focht, {Caroline M.} and Groziak, {Amanda R.} and Jesse Kao and Kottapalli, {Jagdeesh S.} and Leong, {Matthew C.} and Lin, {Joy J.} and Regina Liu and Luo, {Joanna E.} and Meyer, {Christine M.} and Mo, {Albert F.} and Pahng, {Seong Ho} and Vinay Penna and Raciti, {Chris D.} and Abhinav Srinath and Shwetha Sudhakar and Tang, {Joseph D.} and Cox, {Brian R.} and Holland, {Cynthia K.} and Barrie Cascella and Wilhelm Cruz and McClerkin, {Sheri A.} and Kunkel, {Barbara N.} and Jez, {Joseph M.}",
note = "Funding Information: Funding and additional information—This work was supported by National Science Foundation Grants IOS-1645908 (to B. N. K.) and MCB-1614539 (to J. M. J.) and a grant from the Howard Hughes Medical Institute Science Education Program (to J. M. J.). C.K.H. was supported by National Science Foundation Graduate Research Fellowship DGE-1143954. This project was part of Bio4522, an upper-level laboratory course at Washington University in St. Louis. Portions of this research were carried out at the Argonne National Laboratory Structural Biology Center of the Advanced Photon Source, a national user facility operated by the University of Chicago for the Department of Energy Office of Biological and Environmental Research under Grant DE-AC02-06CH11357. Publisher Copyright: {\textcopyright} 2020 American Society for Biochemistry and Molecular Biology Inc.. All rights reserved.",
year = "2020",
month = oct,
day = "2",
doi = "10.1074/jbc.RA120.014747",
language = "English",
volume = "295",
pages = "13914--13926",
journal = "Journal of Biological Chemistry",
issn = "0021-9258",
number = "40",
}