TY - JOUR
T1 - The pheromone darcin drives a circuit for innate and reinforced behaviours
AU - Demir, Ebru
AU - Li, Kenneth
AU - Bobrowski-Khoury, Natasha
AU - Sanders, Joshua I.
AU - Beynon, Robert J.
AU - Hurst, Jane L.
AU - Kepecs, Adam
AU - Axel, Richard
N1 - Funding Information:
Acknowledgements We thank Z. J. Huang, R. Paik, H. Taniguchi, G. Enikopolov, S. P. Ranade and D. Kvitsiani for discussions; L. McLean for assistance with recombinant proteins; R. Eifert, B. Burbach and R. Specht for technical support; S. Brenner-Morton for generating the guinea pig Fos antibody; K. Chatpar and Y. Sun for assistance with experiments; N. Zabello for help with mice; L. Stowers for the cat lipocalin (Fel-D4) plasmid; C. Denny for the gift of the Arc-CreER mouse; D. Hattori, J. Scribner, A. S. Lee and B. Noro for comments on the manuscript; and C. H. Eccard, P. Kisloff, A. Nemes and M. Gutierrez for laboratory support. This work was supported by the Howard Hughes Medical Institute (R.A.), the Biotechnology and Biological Sciences Research Council (J.L.H. and R.J.B.), and The Robert E. Leet and Clara Guthrie Patterson Trust Fellowship (E.D.).
Publisher Copyright:
© 2020, The Author(s), under exclusive licence to Springer Nature Limited.
PY - 2020/2/6
Y1 - 2020/2/6
N2 - Organisms have evolved diverse behavioural strategies that enhance the likelihood of encountering and assessing mates1. Many species use pheromones to communicate information about the location, sexual and social status of potential partners2. In mice, the major urinary protein darcin—which is present in the urine of males—provides a component of a scent mark that elicits approach by females and drives learning3,4. Here we show that darcin elicits a complex and variable behavioural repertoire that consists of attraction, ultrasonic vocalization and urinary scent marking, and also serves as a reinforcer in learning paradigms. We identify a genetically determined circuit—extending from the accessory olfactory bulb to the posterior medial amygdala—that is necessary for all behavioural responses to darcin. Moreover, optical activation of darcin-responsive neurons in the medial amygdala induces both the innate and the conditioned behaviours elicited by the pheromone. These neurons define a topographically segregated population that expresses neuronal nitric oxide synthase. We suggest that this darcin-activated neural circuit integrates pheromonal information with internal state to elicit both variable innate behaviours and reinforced behaviours that may promote mate encounters and mate selection.
AB - Organisms have evolved diverse behavioural strategies that enhance the likelihood of encountering and assessing mates1. Many species use pheromones to communicate information about the location, sexual and social status of potential partners2. In mice, the major urinary protein darcin—which is present in the urine of males—provides a component of a scent mark that elicits approach by females and drives learning3,4. Here we show that darcin elicits a complex and variable behavioural repertoire that consists of attraction, ultrasonic vocalization and urinary scent marking, and also serves as a reinforcer in learning paradigms. We identify a genetically determined circuit—extending from the accessory olfactory bulb to the posterior medial amygdala—that is necessary for all behavioural responses to darcin. Moreover, optical activation of darcin-responsive neurons in the medial amygdala induces both the innate and the conditioned behaviours elicited by the pheromone. These neurons define a topographically segregated population that expresses neuronal nitric oxide synthase. We suggest that this darcin-activated neural circuit integrates pheromonal information with internal state to elicit both variable innate behaviours and reinforced behaviours that may promote mate encounters and mate selection.
UR - http://www.scopus.com/inward/record.url?scp=85078679607&partnerID=8YFLogxK
U2 - 10.1038/s41586-020-1967-8
DO - 10.1038/s41586-020-1967-8
M3 - Article
C2 - 31996852
AN - SCOPUS:85078679607
SN - 0028-0836
VL - 578
SP - 137
EP - 141
JO - Nature
JF - Nature
IS - 7793
ER -