The Pel polysaccharide is predominantly composed of a dimeric repeat of α-1,4 linked galactosamine and N-acetylgalactosamine

François Le Mauff, Erum Razvi, Courtney Reichhardt, Piyanka Sivarajah, Matthew R. Parsek, P. Lynne Howell, Donald C. Sheppard

Research output: Contribution to journalArticlepeer-review

21 Scopus citations

Abstract

The genetic capacity to synthesize the biofilm matrix exopolysaccharide Pel is widespread among Gram-negative and Gram-positive bacteria. However, its exact chemical structure has been challenging to determine. Using a Pseudomonas aeruginosa strain engineered to overproduce Pel, improvements to the isolation procedure, and selective hydrolysis with the glycoside hydrolase PelAh, we demonstrate that Pel is a partially de-N-acetylated linear polymer of α-1,4-N-acetylgalactosamine comprised predominantly of dimeric repeats of galactosamine and N-acetylgalactosamine.

Original languageEnglish
Article number502
JournalCommunications Biology
Volume5
Issue number1
DOIs
StatePublished - Dec 2022

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