We have investigated the ontogeny of, and the effect of hypophysectomy on, immunoreactive β-endorphin in rat ovaries. Total levels rose with ovarian weight from nondetectable levels at 5 days of age to approximately 0.15 pmol/ovary at 80 days; thereafter, the levels remained constant through 201 days of age. Hypophysectomy decreased both ovarian weight and the total content of immunoreactive β-endorphin, but the concentration per weight was not significantly altered. Most of the immunoreactive β-endorphin before puberty chromatographed like authentic β-endorphin, but after puberty most chromatographed like β-lipotropin. Hypophysectomy did not alter this chromatographic pattern.
|Number of pages||6|
|Journal||Biochemical and Biophysical Research Communications|
|State||Published - Mar 31 1989|