TY - JOUR
T1 - The next generation of ocular pathogen detection
AU - Sabapathypillai, Sharon L.
AU - James, Hayley R.
AU - Lyerla, Rachael R.L.
AU - Hassman, Lynn
N1 - Funding Information:
Submitted September 17, 2020; accepted November 30, 2020. From the Department of Ophthalmology and Visual Sciences, Washington Uni-versity in St. Louis, St. Louis, MO. This work was supported by an unrestricted grant from Research to Prevent Blindness. The authors have no conflicts of interest to disclose. Address correspondence and reprint requests to: Lynn Hassman, Campus Box 8096, 660 S Euclid Ave, St. Louis, MO 63110. E-mail: lhassman@wustl.edu. Copyright © 2021 Asia-Pacific Academy of Ophthalmology. Published by Wolters Kluwer Health, Inc. on behalf of the Asia-Pacific Academy of Ophthalmology. This is an open access article distributed under the terms of the Creative Commons Attribution-Non Commercial-No Derivatives License 4.0 (CCBY-NC-ND), where it is permissible to download and share the work provided it is properly cited. The work cannot be changed in any way or used commercially without permission from the journal. ISSN: 2162-0989 DOI: 10.1097/APO.0000000000000366
Publisher Copyright:
©2021 Asia-Pacific Academy of Ophthalmology.
PY - 2021/1
Y1 - 2021/1
N2 - Metagenomic next-generation sequencing is a powerful method for pathogen detection that combines advanced genome sequencing technology with cutting-edge bioinformatics to analyze microbial populations. Metagenomic next-generation sequencing has the potential to identify uncommon, unculturable, and even previously unidentified pathogens from a clinical isolate. Of particular interest to ophthalmology, this robust data extraction can occur from very small volume clinical samples. Here we discuss the opportunities and limitations of this technique and their current and future application to ophthalmic diagnostics.
AB - Metagenomic next-generation sequencing is a powerful method for pathogen detection that combines advanced genome sequencing technology with cutting-edge bioinformatics to analyze microbial populations. Metagenomic next-generation sequencing has the potential to identify uncommon, unculturable, and even previously unidentified pathogens from a clinical isolate. Of particular interest to ophthalmology, this robust data extraction can occur from very small volume clinical samples. Here we discuss the opportunities and limitations of this technique and their current and future application to ophthalmic diagnostics.
KW - Endophthalmitis
KW - Next-generation sequencing
KW - Uveitis
UR - http://www.scopus.com/inward/record.url?scp=85100607712&partnerID=8YFLogxK
U2 - 10.1097/APO.0000000000000366
DO - 10.1097/APO.0000000000000366
M3 - Review article
C2 - 33512832
AN - SCOPUS:85100607712
SN - 2162-0989
VL - 10
SP - 109
EP - 113
JO - Asia-Pacific Journal of Ophthalmology
JF - Asia-Pacific Journal of Ophthalmology
IS - 1
ER -