The NEMP family supports metazoan fertility and nuclear envelope stiffness

Yonit Tsatskis; Robyn Rosenfeld; Joel D. Pearson; Curtis Boswell; Yi Qu; Kyunga Kim; Lacramioara Fabian; Ariz Mohammad; Xian Wang; Michael I. Robson; Karen Krchma; Jun Wu; João Gonçalves; Didier Hodzic; Shu Wu; Daniel Potter; Laurence Pelletier; Wade H. Dunham; Anne Claude Gingras; Yu Sun; Jin Meng; Dorothea Godt; Tim Schedl; Brian Ciruna; Kyunghee Choi; John R.B. Perry; Rod Bremner; Eric C. Schirmer; Julie A. Brill; Andrea Jurisicova; Helen McNeill

doi:10.1126/sciadv.abb4591

The NEMP family supports metazoan fertility and nuclear envelope stiffness

Yonit Tsatskis, Robyn Rosenfeld, Joel D. Pearson, Curtis Boswell, Yi Qu, Kyunga Kim, Lacramioara Fabian, Ariz Mohammad, Xian Wang, Michael I. Robson, Karen Krchma, Jun Wu, João Gonçalves, Didier Hodzic, Shu Wu, Daniel Potter, Laurence Pelletier, Wade H. Dunham, Anne Claude Gingras, Yu SunJin Meng, Dorothea Godt, Tim Schedl, Brian Ciruna, Kyunghee Choi, John R.B. Perry, Rod Bremner, Eric C. Schirmer, Julie A. Brill, Andrea Jurisicova, Helen McNeill

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12 Scopus citations

Abstract

Human genome-wide association studies have linked single-nucleotide polymorphisms (SNPs) in NEMP1 (nuclear envelope membrane protein 1) with early menopause; however, it is unclear whether NEMP1 has any role in fertility. We show that whole-animal loss of NEMP1 homologs in Drosophila, Caenorhabditis elegans, zebrafish, and mice leads to sterility or early loss of fertility. Loss of Nemp leads to nuclear shaping defects, most prominently in the germ line. Biochemical, biophysical, and genetic studies reveal that NEMP proteins support the mechanical stiffness of the germline nuclear envelope via formation of a NEMP-EMERIN complex. These data indicate that the germline nuclear envelope has specialized mechanical properties and that NEMP proteins play essential and conserved roles in fertility.

Original language	English
Article number	eabb4591
Journal	Science Advances
Volume	6
Issue number	35
DOIs	https://doi.org/10.1126/sciadv.abb4591
State	Published - Aug 2020

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Tsatskis, Y., Rosenfeld, R., Pearson, J. D., Boswell, C., Qu, Y., Kim, K., Fabian, L., Mohammad, A., Wang, X., Robson, M. I., Krchma, K., Wu, J., Gonçalves, J., Hodzic, D., Wu, S., Potter, D., Pelletier, L., Dunham, W. H., Gingras, A. C., ... McNeill, H. (2020). The NEMP family supports metazoan fertility and nuclear envelope stiffness. Science Advances, 6(35), Article eabb4591. https://doi.org/10.1126/sciadv.abb4591

@article{82cfe5e02b60458781b6abf1c830a287,

title = "The NEMP family supports metazoan fertility and nuclear envelope stiffness",

abstract = "Human genome-wide association studies have linked single-nucleotide polymorphisms (SNPs) in NEMP1 (nuclear envelope membrane protein 1) with early menopause; however, it is unclear whether NEMP1 has any role in fertility. We show that whole-animal loss of NEMP1 homologs in Drosophila, Caenorhabditis elegans, zebrafish, and mice leads to sterility or early loss of fertility. Loss of Nemp leads to nuclear shaping defects, most prominently in the germ line. Biochemical, biophysical, and genetic studies reveal that NEMP proteins support the mechanical stiffness of the germline nuclear envelope via formation of a NEMP-EMERIN complex. These data indicate that the germline nuclear envelope has specialized mechanical properties and that NEMP proteins play essential and conserved roles in fertility.",

author = "Yonit Tsatskis and Robyn Rosenfeld and Pearson, {Joel D.} and Curtis Boswell and Yi Qu and Kyunga Kim and Lacramioara Fabian and Ariz Mohammad and Xian Wang and Robson, {Michael I.} and Karen Krchma and Jun Wu and Jo{\~a}o Gon{\c c}alves and Didier Hodzic and Shu Wu and Daniel Potter and Laurence Pelletier and Dunham, {Wade H.} and Gingras, {Anne Claude} and Yu Sun and Jin Meng and Dorothea Godt and Tim Schedl and Brian Ciruna and Kyunghee Choi and Perry, {John R.B.} and Rod Bremner and Schirmer, {Eric C.} and Brill, {Julie A.} and Andrea Jurisicova and Helen McNeill",

note = "Publisher Copyright: Copyright {\textcopyright} 2020 The Authors, some rights reserved.",

year = "2020",

month = aug,

doi = "10.1126/sciadv.abb4591",

language = "English",

volume = "6",

journal = "Science Advances",

issn = "2375-2548",

number = "35",

}

Tsatskis, Y, Rosenfeld, R, Pearson, JD, Boswell, C, Qu, Y, Kim, K, Fabian, L, Mohammad, A, Wang, X, Robson, MI, Krchma, K, Wu, J, Gonçalves, J, Hodzic, D, Wu, S, Potter, D, Pelletier, L, Dunham, WH, Gingras, AC, Sun, Y, Meng, J, Godt, D, Schedl, T, Ciruna, B, Choi, K, Perry, JRB, Bremner, R, Schirmer, EC, Brill, JA, Jurisicova, A & McNeill, H 2020, 'The NEMP family supports metazoan fertility and nuclear envelope stiffness', Science Advances, vol. 6, no. 35, eabb4591. https://doi.org/10.1126/sciadv.abb4591

TY - JOUR

T1 - The NEMP family supports metazoan fertility and nuclear envelope stiffness

AU - Tsatskis, Yonit

AU - Rosenfeld, Robyn

AU - Pearson, Joel D.

AU - Boswell, Curtis

AU - Qu, Yi

AU - Kim, Kyunga

AU - Fabian, Lacramioara

AU - Mohammad, Ariz

AU - Wang, Xian

AU - Robson, Michael I.

AU - Krchma, Karen

AU - Wu, Jun

AU - Gonçalves, João

AU - Hodzic, Didier

AU - Wu, Shu

AU - Potter, Daniel

AU - Pelletier, Laurence

AU - Dunham, Wade H.

AU - Gingras, Anne Claude

AU - Sun, Yu

AU - Meng, Jin

AU - Godt, Dorothea

AU - Schedl, Tim

AU - Ciruna, Brian

AU - Choi, Kyunghee

AU - Perry, John R.B.

AU - Bremner, Rod

AU - Schirmer, Eric C.

AU - Brill, Julie A.

AU - Jurisicova, Andrea

AU - McNeill, Helen

PY - 2020/8

Y1 - 2020/8

N2 - Human genome-wide association studies have linked single-nucleotide polymorphisms (SNPs) in NEMP1 (nuclear envelope membrane protein 1) with early menopause; however, it is unclear whether NEMP1 has any role in fertility. We show that whole-animal loss of NEMP1 homologs in Drosophila, Caenorhabditis elegans, zebrafish, and mice leads to sterility or early loss of fertility. Loss of Nemp leads to nuclear shaping defects, most prominently in the germ line. Biochemical, biophysical, and genetic studies reveal that NEMP proteins support the mechanical stiffness of the germline nuclear envelope via formation of a NEMP-EMERIN complex. These data indicate that the germline nuclear envelope has specialized mechanical properties and that NEMP proteins play essential and conserved roles in fertility.

AB - Human genome-wide association studies have linked single-nucleotide polymorphisms (SNPs) in NEMP1 (nuclear envelope membrane protein 1) with early menopause; however, it is unclear whether NEMP1 has any role in fertility. We show that whole-animal loss of NEMP1 homologs in Drosophila, Caenorhabditis elegans, zebrafish, and mice leads to sterility or early loss of fertility. Loss of Nemp leads to nuclear shaping defects, most prominently in the germ line. Biochemical, biophysical, and genetic studies reveal that NEMP proteins support the mechanical stiffness of the germline nuclear envelope via formation of a NEMP-EMERIN complex. These data indicate that the germline nuclear envelope has specialized mechanical properties and that NEMP proteins play essential and conserved roles in fertility.

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U2 - 10.1126/sciadv.abb4591

DO - 10.1126/sciadv.abb4591

M3 - Article

C2 - 32923640

AN - SCOPUS:85090872373

SN - 2375-2548

VL - 6

JO - Science Advances

JF - Science Advances

IS - 35

M1 - eabb4591

ER -

The NEMP family supports metazoan fertility and nuclear envelope stiffness

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