@article{82cfe5e02b60458781b6abf1c830a287,
title = "The NEMP family supports metazoan fertility and nuclear envelope stiffness",
abstract = "Human genome-wide association studies have linked single-nucleotide polymorphisms (SNPs) in NEMP1 (nuclear envelope membrane protein 1) with early menopause; however, it is unclear whether NEMP1 has any role in fertility. We show that whole-animal loss of NEMP1 homologs in Drosophila, Caenorhabditis elegans, zebrafish, and mice leads to sterility or early loss of fertility. Loss of Nemp leads to nuclear shaping defects, most prominently in the germ line. Biochemical, biophysical, and genetic studies reveal that NEMP proteins support the mechanical stiffness of the germline nuclear envelope via formation of a NEMP-EMERIN complex. These data indicate that the germline nuclear envelope has specialized mechanical properties and that NEMP proteins play essential and conserved roles in fertility.",
author = "Yonit Tsatskis and Robyn Rosenfeld and Pearson, {Joel D.} and Curtis Boswell and Yi Qu and Kyunga Kim and Lacramioara Fabian and Ariz Mohammad and Xian Wang and Robson, {Michael I.} and Karen Krchma and Jun Wu and Jo{\~a}o Gon{\c c}alves and Didier Hodzic and Shu Wu and Daniel Potter and Laurence Pelletier and Dunham, {Wade H.} and Gingras, {Anne Claude} and Yu Sun and Jin Meng and Dorothea Godt and Tim Schedl and Brian Ciruna and Kyunghee Choi and Perry, {John R.B.} and Rod Bremner and Schirmer, {Eric C.} and Brill, {Julie A.} and Andrea Jurisicova and Helen McNeill",
note = "Funding Information: Financial support for this work was provided by Canadian Institutes of Health Research grants 143319 to H.M., MOP-42462 and PJT-148658 to B.C., 153128 to R.B., 156081 to A.J., MOP-102546 and MOP-130437 to J.A.B., 143301 to A.-C.G., and 167279 to L.P. This work was also supported, in part, by the Krembil Foundation to L.P., the Canada Research Chair program to B.C., NIH R01 GM100756 to T.S., NSERC Discovery grant to D.G. and Y.S., NIH to K.C., and the Medical Research Council (unit programme MC_UU_12015/2) to J.R.B.P. Financial support was also provided by the Wellcome Senior Research Fellowship 095209 to E.C.S., Core funding 092076 to the Wellcome Centre for Cell Biology, and a Wellcome studentship to M.I.R. Ontario Research Funds—Research Excellence Program were provided to Y.S. Proteomics work was performed at the Network Biology Collaborative Centre at the Lunenfeld-Tanenbaum Research Institute, a facility supported by Canada Foundation for Innovation funding, by the Ontarian Government, and by the Genome Canada and Ontario Genomics (OGI-097 and OGI-139) Publisher Copyright: Copyright {\textcopyright} 2020 The Authors, some rights reserved.",
year = "2020",
month = aug,
doi = "10.1126/sciadv.abb4591",
language = "English",
volume = "6",
journal = "Science Advances",
issn = "2375-2548",
number = "35",
}