Chick embryo calvaria RNA has been translated in a nucleasetreated reticulocyte lysate system. The products have been characterized by their susceptibility to collagenase digestion and precipitability by Type I procollagen chain antibodies. The largest product, pre procollagen αl(I), migrates more slowly than proto procollagen αl(I) isolated from calvaria, contains initiator methionine and is cleaved to proto procollagen αl(I) by dog pancreas microsomes. The molecular weight of pre procollagen αl(I) was estimated to be 165,000 and by a similar analysis, pre procollagen α2 was identified and its weight determined to be approximately 165,000 daltons. Bacterial collagenase digestion of pre procollagen αl(I) generated two new low molecular weight hydrophobic components.
|Number of pages||9|
|Journal||Biochemical and Biophysical Research Communications|
|State||Published - Jan 29 1980|