TY - JOUR
T1 - The molecular weight of the cell-free translation product of αl(I) procollagen mRNA
AU - Sandell, Linda
AU - Veis, Arthur
N1 - Funding Information:
by NIH Grant and Digestive
PY - 1980/1/29
Y1 - 1980/1/29
N2 - Chick embryo calvaria RNA has been translated in a nucleasetreated reticulocyte lysate system. The products have been characterized by their susceptibility to collagenase digestion and precipitability by Type I procollagen chain antibodies. The largest product, pre procollagen αl(I), migrates more slowly than proto procollagen αl(I) isolated from calvaria, contains initiator methionine and is cleaved to proto procollagen αl(I) by dog pancreas microsomes. The molecular weight of pre procollagen αl(I) was estimated to be 165,000 and by a similar analysis, pre procollagen α2 was identified and its weight determined to be approximately 165,000 daltons. Bacterial collagenase digestion of pre procollagen αl(I) generated two new low molecular weight hydrophobic components.
AB - Chick embryo calvaria RNA has been translated in a nucleasetreated reticulocyte lysate system. The products have been characterized by their susceptibility to collagenase digestion and precipitability by Type I procollagen chain antibodies. The largest product, pre procollagen αl(I), migrates more slowly than proto procollagen αl(I) isolated from calvaria, contains initiator methionine and is cleaved to proto procollagen αl(I) by dog pancreas microsomes. The molecular weight of pre procollagen αl(I) was estimated to be 165,000 and by a similar analysis, pre procollagen α2 was identified and its weight determined to be approximately 165,000 daltons. Bacterial collagenase digestion of pre procollagen αl(I) generated two new low molecular weight hydrophobic components.
UR - http://www.scopus.com/inward/record.url?scp=0019334851&partnerID=8YFLogxK
U2 - 10.1016/0006-291X(80)90369-1
DO - 10.1016/0006-291X(80)90369-1
M3 - Article
C2 - 7356481
AN - SCOPUS:0019334851
SN - 0006-291X
VL - 92
SP - 554
EP - 562
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 2
ER -