The matricellular protein SPARC induces inflammatory interferon-response in macrophages during aging

Seungjin Ryu, Sviatoslav Sidorov, Eric Ravussin, Maxim Artyomov, Akiko Iwasaki, Andrew Wang, Vishwa Deep Dixit

Research output: Contribution to journalArticlepeer-review

12 Scopus citations


The risk of chronic diseases caused by aging is reduced by caloric restriction (CR)-induced immunometabolic adaptation. Here, we found that the matricellular protein, secreted protein acidic and rich in cysteine (SPARC), was inhibited by 2 years of 14% sustained CR in humans and elevated by obesity. SPARC converted anti-inflammatory macrophages into a pro-inflammatory phenotype with induction of interferon-stimulated gene (ISG) expression via the transcription factors IRF3/7. Mechanistically, SPARC-induced ISGs were dependent on toll-like receptor-4 (TLR4)-mediated TBK1, IRF3, IFN-β, and STAT1 signaling without engaging the Myd88 pathway. Metabolically, SPARC dampened mitochondrial respiration, and inhibition of glycolysis abrogated ISG induction by SPARC in macrophages. Furthermore, the N-terminal acidic domain of SPARC was required for ISG induction, while adipocyte-specific deletion of SPARC reduced inflammation and extended health span during aging. Collectively, SPARC, a CR-mimetic adipokine, is an immunometabolic checkpoint of inflammation and interferon response that may be targeted to delay age-related metabolic and functional decline.

Original languageEnglish
Pages (from-to)1609-1626.e7
Issue number9
StatePublished - Sep 13 2022


  • TLR4
  • caloric restriction
  • inflammation
  • interferon-stimulated gene
  • macrophage
  • matricellular protein


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