The mannose receptor functions as a high capacity and broad specificity antigen receptor in human dendritic cells

Anneke J. Engering, Marina Cella, Donna Fluitsma, Manfred Brockhaus, Elizabeth C.M. Hoefsmit, Antonio Lanzavecchia, Jean Pieters

Research output: Contribution to journalArticlepeer-review

340 Scopus citations

Abstract

Dendritic cells, in contrast to B lymphocytes, must be able to efficiently internalize a diverse array of antigens for processing and loading onto major histocompatibility complex (MHC) class II molecules. Here we characterize the mannose receptor pathway in dendritic cells and show that mannose receptor-mediated uptake of antigens results in a ~ 100-fold more efficient presentation to T cells, as compared to antigens internalized via fluid phase. Immunocytochemistry as well as subcellular fractionation revealed the localization of the mannose receptor and MHC class II molecules in distinct subcellular compartments. The mannose receptor thus functions in rapid internalization and concentration of a variety of glycosylated antigens that become available for processing and presentation. This may contribute to the unique capacity of dendritic cells to generate primary T cell responses against infectious agents.

Original languageEnglish
Pages (from-to)2417-2425
Number of pages9
JournalEuropean Journal of Immunology
Volume27
Issue number9
DOIs
StatePublished - Sep 1 1997

Keywords

  • Antigen internalization
  • Dendritic cell
  • Mannose receptor

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