The major delayed rectifier in both Drosophila neurons and muscle is encoded by Shab

S. Tsunoda, L. Salkoff

Research output: Contribution to journalArticlepeer-review

64 Scopus citations

Abstract

The delayed rectifier K+ current in Drosophila is similar to the classical delayed rectifier, originally described by Hodgkin and Huxley. Drosophila provides unique tools of mutant analysis to unambiguously determine the genetic identity of this native K+ current. We identified the Shab gene as the exclusive gene underlying delayed rectifier currents in both muscle and neurons. In muscles, a genetic mutation of Shab removes virtually all the whole cell delayed rectifier current (I(K)), while leaving unaltered the transient A-current encoded by the Shaker gene. In neurons, the Shab mutation also removes the bulk of I(K), but leaves unaltered the transient A- current encoded by the ShaI gene. Although most of the delayed rectifier current is the product of the Shab gene, the Shaw gene contributes a small 'leak' current to most neurons and muscle cells. Thus, in contrast to the A- currents which are encoded by different genes in muscle and neuronal cell bodies (Shaker and ShaI, respectively), the predominant I(K) in both muscle and neurons is encoded by the same gene (Shab). With the genetic identity of I(K) confirmed, all of the major K+ currents in embryonic Drosophila neurons and muscle are now known.

Original languageEnglish
Pages (from-to)5209-5221
Number of pages13
JournalJournal of Neuroscience
Volume15
Issue number7 II
DOIs
StatePublished - 1995

Keywords

  • Drosophila
  • I(K)
  • ShaI
  • Shab
  • Shaker
  • Shaw
  • delayed rectifier
  • embryonic neurons
  • excitable membranes
  • mutant
  • mutant analysis
  • potassium channels

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