The low-density lipoprotein receptor-related protein (LRP) mediates clearance of coagulation factor Xa in vivo

Masaaki Narita, Amy E. Rudolph, Joseph P. Miletich, Alan L. Schwartz

Research output: Contribution to journalArticle

37 Scopus citations

Abstract

Blood coagulation factor X plays a pivotal role in the clotting cascade. When administered intravenously to mice, the majority of activated factor X (factor Xa) binds to αΥ macroglobulin (α2M) and is rapidly cleared from the circulation into liver. We show here that the low-density lipoprotein receptor-related protein (LRP) is responsible for factor Xa catabolism in vivo. Mica overexpressing a 39-kD receptor-associated protein that binds to LRP and inhibits its ligand binding activity displayed dramatically prolonged plasma clearance of 125I-factor Xa. Preadministration of α2M-proteinase complexes (α2M*) also diminished the plasma clearance of 125I-factor Xa in a dose-dependent fashion. The clearance of preformed complexes of 125I- factor Xa and α2M was similar to that of 125I-factor Xa alone and was also inhibited by mice overexpressing a 39-kD receptor-associated protein. These results thus suggest that, in vivo, factor Xa is metabolized via LRP after complex formation with α2M.

Original languageEnglish
Pages (from-to)555-560
Number of pages6
JournalBlood
Volume91
Issue number2
DOIs
StatePublished - Jan 15 1998
Externally publishedYes

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