@article{5931d381b04c4ace9b9ead8af3c1e06c,
title = "The lncRNA CASC15 regulates SOX4 expression in RUNX1-rearranged acute leukemia",
abstract = "Background: Long non-coding RNAs (lncRNAs) play a variety of cellular roles, including regulation of transcription and translation, leading to alterations in gene expression. Some lncRNAs modulate the expression of chromosomally adjacent genes. Here, we assess the roles of the lncRNA CASC15 in regulation of a chromosomally nearby gene, SOX4, and its function in RUNX1/AML translocated leukemia. Results: CASC15 is a conserved lncRNA that was upregulated in pediatric B-acute lymphoblastic leukemia (B-ALL) with t (12; 21) as well as pediatric acute myeloid leukemia (AML) with t (8; 21), both of which are associated with relatively better prognosis. Enforced expression of CASC15 led to a myeloid bias in development, and overall, decreased engraftment and colony formation. At the cellular level, CASC15 regulated cellular survival, proliferation, and the expression of its chromosomally adjacent gene, SOX4. Differentially regulated genes following CASC15 knockdown were enriched for predicted transcriptional targets of the Yin and Yang-1 (YY1) transcription factor. Interestingly, we found that CASC15 enhances YY1-mediated regulation of the SOX4 promoter. Conclusions: Our findings represent the first characterization of this CASC15 in RUNX1-translocated leukemia, and point towards a mechanistic basis for its action.",
keywords = "B-all, CASC15, ETV6-RUNX1, Non-coding RNA, SOX4",
author = "Fernando, {Thilini R.} and Contreras, {Jorge R.} and Matteo Zampini and Rodriguez-Malave, {Norma I.} and Alberti, {Michael O.} and Jaime Anguiano and Tran, {Tiffany M.} and Palanichamy, {Jayanth K.} and Jasmine Gajeton and Ung, {Nolan M.} and Aros, {Cody J.} and Waters, {Ella V.} and David Casero and Giuseppe Basso and Martina Pigazzi and Rao, {Dinesh S.}",
note = "Funding Information: We would like to thank the UCLA Clinical Microarray Core for performing the microarray hybridization experiments. We thank Lulan Wang, Parth C. Patel, Katherine Harmeyer, Jennifer King, and May Paing for their input. Flow cytometry was performed in the UCLA Jonsson Comprehensive Cancer Center (JCCC) and Center for AIDS Research Flow Cytometry Core Facility that is supported by National Institutes of Health awards CA-16042 and AI-28697, and by the JCCC, the UCLA AIDS Institute, the UCLA Council of Bioscience Resources, and the David Geffen School of Medicine at UCLA. Funding Information: This research was supported by the NIH T32 CA009056 (T.R.F. and T.M.T.), NIH T32 CA009120 (J.R.C.), NIH T32 HL086345 (N.M.U), National Science Foundation DGE-1144087 (N.I.R.M.), Eugene V. Cota-Robles Fellowship from UCLA (J.R.C. and N.I.R.M.), Eli and Edythe Broad Center of Regenerative Medicine and Stem Cell Research at UCLA Training Program (M.O.A.), NIH Small Research Grant Program R03CA175426 (D.S.R.), the Irving Feintech Family Foundation/ Tower Cancer Research Foundation Research Grant (D.S.R.), the University of California Cancer Research Coordinating Committee (D.S.R.) and the Stein-Oppenheimer Endowment Award (D.S.R.). Publisher Copyright: {\textcopyright} 2017 The Author(s).",
year = "2017",
month = jul,
day = "19",
doi = "10.1186/s12943-017-0692-x",
language = "English",
volume = "16",
journal = "Molecular Cancer",
issn = "1476-4598",
number = "1",
}