TY - JOUR
T1 - The lack of consensus for I-Ag7-peptide binding motifs
T2 - Is there a requirement for anchor amino acid side chains?
AU - Carrasco-Marin, Eugenio
AU - Kanagawa, Osami
AU - Unanue, Emil R.
PY - 1999/7/20
Y1 - 1999/7/20
N2 - We discuss here the problems in identifying sequence motifs of peptides that bind to I-Ag7, the class II histocompatibility molecule of NOD diabetic mice. We present studies that indicate a minor contribution of amino acid side chains for binding. A peptide from the Eα chain binds to I-Ag7 molecules and is recognized by CD4 T cells. By producing single-residue mutations we identified four residues that were considered to contact the T cell receptor. No residue was found to be essential for binding to I-Ag7: a peptide that contained the T cell contact residues, on a backbone of alanines, bound to I-Ag7 and stimulated the T cells. We conclude that peptides can bind to I-Ag7 without the requirement for residues with prominent side chains to anchor them.
AB - We discuss here the problems in identifying sequence motifs of peptides that bind to I-Ag7, the class II histocompatibility molecule of NOD diabetic mice. We present studies that indicate a minor contribution of amino acid side chains for binding. A peptide from the Eα chain binds to I-Ag7 molecules and is recognized by CD4 T cells. By producing single-residue mutations we identified four residues that were considered to contact the T cell receptor. No residue was found to be essential for binding to I-Ag7: a peptide that contained the T cell contact residues, on a backbone of alanines, bound to I-Ag7 and stimulated the T cells. We conclude that peptides can bind to I-Ag7 without the requirement for residues with prominent side chains to anchor them.
UR - http://www.scopus.com/inward/record.url?scp=0033587774&partnerID=8YFLogxK
U2 - 10.1073/pnas.96.15.8621
DO - 10.1073/pnas.96.15.8621
M3 - Article
C2 - 10411925
AN - SCOPUS:0033587774
VL - 96
SP - 8621
EP - 8626
JO - Proceedings of the National Academy of Sciences of the United States of America
JF - Proceedings of the National Academy of Sciences of the United States of America
SN - 0027-8424
IS - 15
ER -