TY - JOUR
T1 - The isolation of mRNA encoding the alpha subunit of human chorionic gonadotropin
AU - Landefeld, Thomas D.
AU - McWilliams, Diana R.
AU - Boime, Irving
N1 - Funding Information:
Purified hCG and its subunits were prepared by Dr. R. E. Canfield through a contract with the center for Population Research, National Institute of Child Health and Human Development, Bethesda, Maryland. This work was supported by U.S. Public Health Service Research Grants PO-HD-08235 and TOI-HD-00379-02.
PY - 1976/9/20
Y1 - 1976/9/20
N2 - MRNA was prepared from first trimester placentae and translated in a wheat germ cell-free system. A major synthesized product had an apparent molecular weight of about 16,000. Tryptic fingerprint analysis revealed that this protein contained peptides overlapping with peptides from authentic alppha subunit of hCG. Since the protein portion of alpha has a molecular weight of 10,600, the data suggest the synthesis of a precursor. No synthesis of the beta subunit was detected. With first trimester RNA, 4-5 times more of the alpha protein was synthesized than with term RNA. Thus, it appears that the larger amounts of hCG-alpha produced in first trimester placenta is due to an increased proportion of the corresponding mRNA.
AB - MRNA was prepared from first trimester placentae and translated in a wheat germ cell-free system. A major synthesized product had an apparent molecular weight of about 16,000. Tryptic fingerprint analysis revealed that this protein contained peptides overlapping with peptides from authentic alppha subunit of hCG. Since the protein portion of alpha has a molecular weight of 10,600, the data suggest the synthesis of a precursor. No synthesis of the beta subunit was detected. With first trimester RNA, 4-5 times more of the alpha protein was synthesized than with term RNA. Thus, it appears that the larger amounts of hCG-alpha produced in first trimester placenta is due to an increased proportion of the corresponding mRNA.
UR - https://www.scopus.com/pages/publications/0017139980
U2 - 10.1016/S0006-291X(76)80054-X
DO - 10.1016/S0006-291X(76)80054-X
M3 - Article
C2 - 985487
AN - SCOPUS:0017139980
SN - 0006-291X
VL - 72
SP - 381
EP - 390
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 2
ER -