The isolation of mRNA encoding the alpha subunit of human chorionic gonadotropin

Thomas D. Landefeld, Diana R. McWilliams, Irving Boime

Research output: Contribution to journalArticlepeer-review

31 Scopus citations


MRNA was prepared from first trimester placentae and translated in a wheat germ cell-free system. A major synthesized product had an apparent molecular weight of about 16,000. Tryptic fingerprint analysis revealed that this protein contained peptides overlapping with peptides from authentic alppha subunit of hCG. Since the protein portion of alpha has a molecular weight of 10,600, the data suggest the synthesis of a precursor. No synthesis of the beta subunit was detected. With first trimester RNA, 4-5 times more of the alpha protein was synthesized than with term RNA. Thus, it appears that the larger amounts of hCG-alpha produced in first trimester placenta is due to an increased proportion of the corresponding mRNA.

Original languageEnglish
Pages (from-to)381-390
Number of pages10
JournalBiochemical and Biophysical Research Communications
Issue number2
StatePublished - Sep 20 1976


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