The isolation and identification of 3,4-dihydroxybenzoic acid formed by nitrogen-fixing Azomonas macrocytogenes

Peter Westervelt, Michael L. Bloom, Gary A. Mabbott, Frank A. Fekete

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11 Scopus citations

Abstract

The mechanism of iron acquisition was studied in nitrogen-fixing Azomonas macrocytogenes. Spent solid agar plating medium samples from Fe-deficient and Fe-sufficient cultures were subjected to the high voltage paper electrophoresis siderophore assay. A yellow-green fluorescent peptide was elicited only under conditions of iron deficiency, whereas a novel dark blue fluorescent, Arnowpositive phenolic compound was detected under both Fe-deficient and Fe-sufficient conditions. Both compounds formed colored complexes with ferric iron. SDS-PAGE analysis of outer envelope protein preparations revealed the hyperproduction of an 83 kDa protein under iron-limiting conditions. Chemical analysis indicated that the phenolate was 3,4-dihydroxybenzoic acid (protocatechuic acid), a compound previously unreported as an extracellular product of a diazotroph.

Original languageEnglish
Pages (from-to)331-335
Number of pages5
JournalFEMS Microbiology Letters
Volume30
Issue number3
DOIs
StatePublished - Dec 1985

Keywords

  • Siderophore
  • iron acquisition
  • iron stress
  • protocatechuic acid

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