TY - JOUR
T1 - The intracellular form of human MAGP1 elicits a complex and specific transcriptional response
AU - Segade, Fernando
AU - Suganuma, Nobuyasu
AU - Mychaleckyj, Josyf C.
AU - Mecham, Robert P.
N1 - Funding Information:
This work was supported by NIH Grant HL071960 (R.P.M.) and by the WFUSM Center for Human Genomics (F.S.). We are grateful to Dax Allred (WFUSM) for his technical support and to Dr. Elisabeth Burton (Penn SDM) for her assistance with microscopy.
PY - 2007
Y1 - 2007
N2 - Microfibril-associated glycoprotein-1 (MAGP1) is found associated with microfibrils in the extracellular matrix (ECM). In humans, MAGP1 is expressed as two alternatively spliced isoforms: MAGP1A, the extracellular microfibril-associated form; and MAGP1B, an exclusively intracellular isoform derived from the skipping of exon 3. The biological function of MAGP1B is unknown. We performed gene expression profiling to study the cellular response to MAGP1B using whole-genome genechips. We found that MAGP1B specifically induces the expression of genes linked to cell adhesion, motility, metabolism, gene expression, development and signal transduction. Versican, a gene product involved in the structure and functional regulation of the ECM, showed the highest up-regulation in response to MAGP1B. These studies suggest a dual role for MAGP1, with extracellular MAGP1A involved in ECM function, and intracellular MAGP1B modulating the expression of genes that function in cell adhesion, migration and control of ECM deposition.
AB - Microfibril-associated glycoprotein-1 (MAGP1) is found associated with microfibrils in the extracellular matrix (ECM). In humans, MAGP1 is expressed as two alternatively spliced isoforms: MAGP1A, the extracellular microfibril-associated form; and MAGP1B, an exclusively intracellular isoform derived from the skipping of exon 3. The biological function of MAGP1B is unknown. We performed gene expression profiling to study the cellular response to MAGP1B using whole-genome genechips. We found that MAGP1B specifically induces the expression of genes linked to cell adhesion, motility, metabolism, gene expression, development and signal transduction. Versican, a gene product involved in the structure and functional regulation of the ECM, showed the highest up-regulation in response to MAGP1B. These studies suggest a dual role for MAGP1, with extracellular MAGP1A involved in ECM function, and intracellular MAGP1B modulating the expression of genes that function in cell adhesion, migration and control of ECM deposition.
KW - Alternate transcripts
KW - Intracrin
KW - MAGP1
KW - Matricellular
KW - Microarray
KW - Microfibrils
UR - http://www.scopus.com/inward/record.url?scp=34748878863&partnerID=8YFLogxK
U2 - 10.1016/j.biocel.2007.06.017
DO - 10.1016/j.biocel.2007.06.017
M3 - Article
C2 - 17692555
AN - SCOPUS:34748878863
VL - 39
SP - 2303
EP - 2313
JO - International Journal of Biochemistry and Cell Biology
JF - International Journal of Biochemistry and Cell Biology
SN - 1357-2725
IS - 12
ER -