Abstract
Tumor necrosis factor (TNF) was cytotoxic at concentrations of 10 to 1000 units/ml to 12 of 14 human tumor cell lines. Synergistic or additive cell killing between TNF and radiation was observed in 7 of 10 tumor cell lines, while independent tumor cell killing by each agent occurred in two tumor cell lines. The maximum synergistic effect was observed when TNF was added 4-12 hr prior to irradiation. This interaction was absent when TNF was added after irradiation. TNF also reduced potentially lethal damage repair in 3 of 5 cell lines tested. Possible mechanisms of interaction of TNF and X rays including induction of hydroxyl radicals and subsequent DNA damage by TNF and radiation are discussed.
Original language | English |
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Pages (from-to) | 69-74 |
Number of pages | 6 |
Journal | International journal of radiation oncology, biology, physics |
Volume | 19 |
Issue number | 1 |
DOIs | |
State | Published - Jul 1990 |
Keywords
- Human tumor cell lines
- Ionizing radiation
- Synergy
- Tumor necrosis factor