The interaction between recombinant human tumor necrosis factor and radiation in 13 human tumor cell lines

Dennis E. Dennis; Michael A. Beckett; Donald Kufe; Ralph R. Weichselbaum

doi:10.1016/0360-3016(90)90136-8

The interaction between recombinant human tumor necrosis factor and radiation in 13 human tumor cell lines

Dennis E. Dennis, Michael A. Beckett, Donald Kufe, Ralph R. Weichselbaum

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98 Scopus citations

Abstract

Tumor necrosis factor (TNF) was cytotoxic at concentrations of 10 to 1000 units/ml to 12 of 14 human tumor cell lines. Synergistic or additive cell killing between TNF and radiation was observed in 7 of 10 tumor cell lines, while independent tumor cell killing by each agent occurred in two tumor cell lines. The maximum synergistic effect was observed when TNF was added 4-12 hr prior to irradiation. This interaction was absent when TNF was added after irradiation. TNF also reduced potentially lethal damage repair in 3 of 5 cell lines tested. Possible mechanisms of interaction of TNF and X rays including induction of hydroxyl radicals and subsequent DNA damage by TNF and radiation are discussed.

Original language	English
Pages (from-to)	69-74
Number of pages	6
Journal	International journal of radiation oncology, biology, physics
Volume	19
Issue number	1
DOIs	https://doi.org/10.1016/0360-3016(90)90136-8
State	Published - Jul 1990

Keywords

Human tumor cell lines
Ionizing radiation
Synergy
Tumor necrosis factor

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10.1016/0360-3016(90)90136-8

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title = "The interaction between recombinant human tumor necrosis factor and radiation in 13 human tumor cell lines",

abstract = "Tumor necrosis factor (TNF) was cytotoxic at concentrations of 10 to 1000 units/ml to 12 of 14 human tumor cell lines. Synergistic or additive cell killing between TNF and radiation was observed in 7 of 10 tumor cell lines, while independent tumor cell killing by each agent occurred in two tumor cell lines. The maximum synergistic effect was observed when TNF was added 4-12 hr prior to irradiation. This interaction was absent when TNF was added after irradiation. TNF also reduced potentially lethal damage repair in 3 of 5 cell lines tested. Possible mechanisms of interaction of TNF and X rays including induction of hydroxyl radicals and subsequent DNA damage by TNF and radiation are discussed.",

keywords = "Human tumor cell lines, Ionizing radiation, Synergy, Tumor necrosis factor",

author = "Dennis, {Dennis E.} and Beckett, {Michael A.} and Donald Kufe and Weichselbaum, {Ralph R.}",

note = "Funding Information: Supported by NC1 grant CA4 1068 and a gift from the Passis Family. Accepted for publication 24 January 1990.",

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T1 - The interaction between recombinant human tumor necrosis factor and radiation in 13 human tumor cell lines

AU - Dennis, Dennis E.

AU - Beckett, Michael A.

AU - Kufe, Donald

AU - Weichselbaum, Ralph R.

N1 - Funding Information: Supported by NC1 grant CA4 1068 and a gift from the Passis Family. Accepted for publication 24 January 1990.

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N2 - Tumor necrosis factor (TNF) was cytotoxic at concentrations of 10 to 1000 units/ml to 12 of 14 human tumor cell lines. Synergistic or additive cell killing between TNF and radiation was observed in 7 of 10 tumor cell lines, while independent tumor cell killing by each agent occurred in two tumor cell lines. The maximum synergistic effect was observed when TNF was added 4-12 hr prior to irradiation. This interaction was absent when TNF was added after irradiation. TNF also reduced potentially lethal damage repair in 3 of 5 cell lines tested. Possible mechanisms of interaction of TNF and X rays including induction of hydroxyl radicals and subsequent DNA damage by TNF and radiation are discussed.

AB - Tumor necrosis factor (TNF) was cytotoxic at concentrations of 10 to 1000 units/ml to 12 of 14 human tumor cell lines. Synergistic or additive cell killing between TNF and radiation was observed in 7 of 10 tumor cell lines, while independent tumor cell killing by each agent occurred in two tumor cell lines. The maximum synergistic effect was observed when TNF was added 4-12 hr prior to irradiation. This interaction was absent when TNF was added after irradiation. TNF also reduced potentially lethal damage repair in 3 of 5 cell lines tested. Possible mechanisms of interaction of TNF and X rays including induction of hydroxyl radicals and subsequent DNA damage by TNF and radiation are discussed.

KW - Human tumor cell lines

KW - Ionizing radiation

KW - Synergy

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The interaction between recombinant human tumor necrosis factor and radiation in 13 human tumor cell lines

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