The impact of antibody protein dose on tumor accumulation of radiolabeled monoclonal antibody was studied in nude mice with xenografts of human melanomas. 225.28S, a murine monoclonal antibody reactive with a high-molecular weight antigen of melanoma, was radiolabeled with I-125 and administered intraperitoneally to nude mice with human melanoma xenografts. Three days later, the animals were sacrificed, and tumor and normal tissue uptake of I-125 antibody was determined. At doses of 6.25, 62.5, 625 and 1875 ug of monoclonal antibody, there were no significant differences in percent of injected dose reaching the tumor/g of tumor or in the non-tumor uptakes achieved. These findings indicate that in the melanoma system, antibody dose is not a critical determinant of tumor uptake, and additionally indicate that low doses of antibody protein are appropriate for studies involving radioiodinated antibody localization.