AIM: To observe the induction on UK by propylene glycol mannite sulfate (PGMS) in rat blood and try to reveal the mechanism of antithrombotic of PGMS on gene level. METHODS: The activity of UK was measured by chromogenic method before and after giving PGMS to rat and the expression of UK mRNA were quantified by semi-quantitative RT-PCR. RESULTS: The UK activity of experimental group and the expression of UK mRNA are higher than that of control group. The increase of UK activity and mRNA expression is in a dose-dependent manner after giving PGMS to rat within the dose of 100mg·kg-1. CONCLUSION: PGMS can induce UK gene expression. This increase of UK gene expression may therefore reflect, in part, an increase in the stability of UK mRNA and/or processing of nuclear UK transcription, which in turn lead to the increase of UK activity in rat blood. As result, PGMS can activate the fibrinolytic system and have function of antithrombotic.
|Number of pages||4|
|Journal||Chinese Pharmacological Bulletin|
|State||Published - Jan 1 2001|
- Propylene glycol mannite sulfate (PGMS)
- Reverse transcription polymerase chain reaction (RT-PCR)
- Urokinase (UK)