TY - JOUR
T1 - The IDA3 adapter, required for intraflagellar transport of I1 dynein, is regulated by ciliary length
AU - Hunter, Emily L.
AU - Lechtreck, Karl
AU - Fu, Gang
AU - Hwang, Juyeon
AU - Lin, Huawen
AU - Gokhale, Avanti
AU - Alford, Lea M.
AU - Lewis, Brian
AU - Yamamoto, Ryosuke
AU - Kamiya, Ritsu
AU - Yang, Fan
AU - Nicastro, Daniela
AU - Dutcher, Susan K.
AU - Wirschell, Maureen
AU - Sale, Winfield S.
AU - Holzbaur, Erika
N1 - Publisher Copyright:
© 2018 Hunter et al.
PY - 2018/4/15
Y1 - 2018/4/15
N2 - Axonemal dyneins, including inner dynein arm I1, assemble in the cytoplasm prior to transport into cilia by intraflagellar transport (IFT). How I1 dynein interacts with IFT is not understood. We take advantage of the Chlamydomonas reinhardtii ida3 mutant, which assembles the inner arm I1 dynein complex in the cytoplasm but fails to transport I1 into the cilium, resulting in I1 dynein-deficient axonemes with abnormal motility. The IDA3 gene encodes an ∼115-kDa coiled-coil protein that primarily enters the cilium during ciliary growth but is not an axonemal protein. During growth, IDA3, along with I1 dynein, is transported by anterograde IFT to the tip of the cilium. At the tip, IDA3 uncouples from IFT and diffuses within the cilium. IFT transport of IDA3 decreases as cilia lengthen and subsides once full length is achieved. IDA3 is the first example of an essential and selective IFT adapter that is regulated by ciliary length.
AB - Axonemal dyneins, including inner dynein arm I1, assemble in the cytoplasm prior to transport into cilia by intraflagellar transport (IFT). How I1 dynein interacts with IFT is not understood. We take advantage of the Chlamydomonas reinhardtii ida3 mutant, which assembles the inner arm I1 dynein complex in the cytoplasm but fails to transport I1 into the cilium, resulting in I1 dynein-deficient axonemes with abnormal motility. The IDA3 gene encodes an ∼115-kDa coiled-coil protein that primarily enters the cilium during ciliary growth but is not an axonemal protein. During growth, IDA3, along with I1 dynein, is transported by anterograde IFT to the tip of the cilium. At the tip, IDA3 uncouples from IFT and diffuses within the cilium. IFT transport of IDA3 decreases as cilia lengthen and subsides once full length is achieved. IDA3 is the first example of an essential and selective IFT adapter that is regulated by ciliary length.
UR - http://www.scopus.com/inward/record.url?scp=85046443183&partnerID=8YFLogxK
U2 - 10.1091/mbc.E17-12-0729
DO - 10.1091/mbc.E17-12-0729
M3 - Article
C2 - 29467251
AN - SCOPUS:85046443183
SN - 1059-1524
VL - 29
SP - 886
EP - 896
JO - Molecular biology of the cell
JF - Molecular biology of the cell
IS - 8
ER -