TY - JOUR
T1 - The Hippo pathway regulates axis formation and morphogenesis in Hydra
AU - Brooun, Maria
AU - Salvenmoser, Willi
AU - Dana, Catherine
AU - Sudol, Marius
AU - Steele, Robert
AU - Hobmayer, Bert
AU - McNeill, Helen
N1 - Funding Information:
ACKNOWLEDGMENTS. We thank Joe Culotti (Lunenfeld Tanenbaum Research Institute, Toronto) for invaluable support of the project and help in writing the manuscript, Leonid Brown (University of Guelph) for help with culturing Hydra, Marina Gertsenstein (Toronto Center for Phenogenomics) for help with the elec-troporation procedure, Thomas Bosch and Alexander Klimovich (University of Kiel) for providing GFP transgenic Hydra, Taylor Skokan (University of California, San Francisco) for providing the MRLC-GFP transgenic Hydra, and Celina Juliano (University of California, Davis) for providing anti-HyWi antibody. H.M. is supported by funding from the Barnes-Jewish/Christian investigator program and is a Larry J. Shapiro and Carol-Ann Uetake-Shapiro Professor. B.H. is supported by the European Commission H2020 Marie Sklodowska-Curie COFUND research grant No. 847681 Ageing, Regeneration and Drug Research. R.S. is supported by Grant 1R24GM080537-01A1 from the National Institute of General Medical Sciences.
Publisher Copyright:
© 2022 by the authors.
PY - 2022/7/19
Y1 - 2022/7/19
N2 - How did cells of early metazoan organisms first organize themselves to form a body axis? The canonical Wnt pathway has been shown to be sufficient for induction of axis in Cnidaria, a sister group to Bilateria, and is important in bilaterian axis formation. Here, we provide experimental evidence that in cnidarian Hydra the Hippo pathway regulates the formation of a new axis during budding upstream of the Wnt pathway. The transcriptional target of the Hippo pathway, the transcriptional coactivator YAP, inhibits the initiation of budding in Hydra and is regulated by Hydra LATS. In addition, we show functions of the Hippo pathway in regulation of actin organization and cell proliferation in Hydra. We hypothesize that the Hippo pathway served as a link between continuous cell division, cell density, and axis formation early in metazoan evolution.
AB - How did cells of early metazoan organisms first organize themselves to form a body axis? The canonical Wnt pathway has been shown to be sufficient for induction of axis in Cnidaria, a sister group to Bilateria, and is important in bilaterian axis formation. Here, we provide experimental evidence that in cnidarian Hydra the Hippo pathway regulates the formation of a new axis during budding upstream of the Wnt pathway. The transcriptional target of the Hippo pathway, the transcriptional coactivator YAP, inhibits the initiation of budding in Hydra and is regulated by Hydra LATS. In addition, we show functions of the Hippo pathway in regulation of actin organization and cell proliferation in Hydra. We hypothesize that the Hippo pathway served as a link between continuous cell division, cell density, and axis formation early in metazoan evolution.
KW - Hippo
KW - Hydra
KW - Wnt
KW - Yap
KW - axis formation
UR - http://www.scopus.com/inward/record.url?scp=85134538253&partnerID=8YFLogxK
U2 - 10.1073/pnas.2203257119
DO - 10.1073/pnas.2203257119
M3 - Article
C2 - 35858299
AN - SCOPUS:85134538253
SN - 0027-8424
VL - 119
JO - Proceedings of the National Academy of Sciences of the United States of America
JF - Proceedings of the National Academy of Sciences of the United States of America
IS - 29
M1 - e2203257119
ER -