TY - JOUR
T1 - The high-mobility-group box protein SSRP1/T160 is essential for cell viability in day 3.5 mouse embryos
AU - Cao, Shang
AU - Bendall, Heather
AU - Hicks, Geoffrey G.
AU - Nashabi, Abudi
AU - Sakano, Hitoshi
AU - Shinkai, Yoichi
AU - Gariglio, Marisa
AU - Oltz, Eugene M.
AU - Ruley, H. Earl
PY - 2003/8
Y1 - 2003/8
N2 - The high-mobility-group (HMG) SSRP1 protein is a member of a conserved chromatin-remodeling complex (FACT/DUF/CP) implicated in DNA replication, basal and regulated transcription, and DNA repair. To assist in the functional analysis of SSRP1, the Ssrp1 gene was targeted in murine embryonic stem cells, and the mutation was introduced into the germ line. Embryos homozygous for the targeted allele die soon after implantation, and preimplantation blastocysts are defective for cell outgrowth and/or survival in vitro. The Ssrp1 mutation was also crossed into a p53 null background without affecting growth and/or survival defects caused by loss of Ssrp1 function. Thus, Ssrp1 appears to encode nonredundant and p53-independent functions that are essential for cell viability.
AB - The high-mobility-group (HMG) SSRP1 protein is a member of a conserved chromatin-remodeling complex (FACT/DUF/CP) implicated in DNA replication, basal and regulated transcription, and DNA repair. To assist in the functional analysis of SSRP1, the Ssrp1 gene was targeted in murine embryonic stem cells, and the mutation was introduced into the germ line. Embryos homozygous for the targeted allele die soon after implantation, and preimplantation blastocysts are defective for cell outgrowth and/or survival in vitro. The Ssrp1 mutation was also crossed into a p53 null background without affecting growth and/or survival defects caused by loss of Ssrp1 function. Thus, Ssrp1 appears to encode nonredundant and p53-independent functions that are essential for cell viability.
UR - https://www.scopus.com/pages/publications/0042091964
U2 - 10.1128/MCB.23.15.5301-5307.2003
DO - 10.1128/MCB.23.15.5301-5307.2003
M3 - Article
C2 - 12861016
AN - SCOPUS:0042091964
SN - 0270-7306
VL - 23
SP - 5301
EP - 5307
JO - Molecular and cellular biology
JF - Molecular and cellular biology
IS - 15
ER -