The genome of the basidiomycetous yeast and human pathogen Cryptococcus neoformans

Brendan J. Loftus; Eula Fung; Paola Roncaglia; Don Rowley; Paolo Amedeo; Dan Bruno; Jessica Vamathevan; Molly Miranda; Iain J. Anderson; James A. Fraser; Jonathan E. Allen; Ian E. Bosdet; Michael R. Brent; Readman Chiu; Tamara L. Doering; Maureen J. Donlin; Cletus A. D'Souza; Deborah S. Fox; Viktoriya Grinberg; Jianmin Fu; Marilyn Fukushima; Brian J. Haas; James C. Huang; Guilhem Janbon; Steven J.M. Jones; Hean L. Koo; Martin I. Krzywinski; June K. Kwon-Chung; Klaus B. Lengeler; Rama Maiti; Marco A. Marra; Robert E. Marra; Carrie A. Mathewson; Thomas G. Mitchell; Mihaela Pertea; Florenta R. Riggs; Steven L. Salzberg; Jacqueline E. Schein; Alla Shvartsbeyn; Heesun Shin; Martin Shumway; Charles A. Specht; Bernard B. Suh; Aaron Tenney; Terry R. Utterback; Brian L. Wickes; Jennifer R. Wortman; Natasja H. Wye; James W. Kronstad; Jennifer K. Lodge; Joseph Heitman; Ronald W. Davis; Claire M. Fraser; Richard W. Hyman

doi:10.1126/science.1103773

The genome of the basidiomycetous yeast and human pathogen Cryptococcus neoformans

Brendan J. Loftus, Eula Fung, Paola Roncaglia, Don Rowley, Paolo Amedeo, Dan Bruno, Jessica Vamathevan, Molly Miranda, Iain J. Anderson, James A. Fraser, Jonathan E. Allen, Ian E. Bosdet, Michael R. Brent, Readman Chiu, Tamara L. Doering, Maureen J. Donlin, Cletus A. D'Souza, Deborah S. Fox, Viktoriya Grinberg, Jianmin FuMarilyn Fukushima, Brian J. Haas, James C. Huang, Guilhem Janbon, Steven J.M. Jones, Hean L. Koo, Martin I. Krzywinski, June K. Kwon-Chung, Klaus B. Lengeler, Rama Maiti, Marco A. Marra, Robert E. Marra, Carrie A. Mathewson, Thomas G. Mitchell, Mihaela Pertea, Florenta R. Riggs, Steven L. Salzberg, Jacqueline E. Schein, Alla Shvartsbeyn, Heesun Shin, Martin Shumway, Charles A. Specht, Bernard B. Suh, Aaron Tenney, Terry R. Utterback, Brian L. Wickes, Jennifer R. Wortman, Natasja H. Wye, James W. Kronstad, Jennifer K. Lodge, Joseph Heitman, Ronald W. Davis, Claire M. Fraser, Richard W. Hyman

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Abstract

Cryptococcus neoformans is a basidiomycetous yeast ubiquitous in the environment, a model for fungal pathogenesis, and an opportunistic human pathogen of global importance. We have sequenced its ∼20-megabase genome, which contains ∼6500 intron-rich gene structures and encodes a transcriptome abundant in alternatively spliced and antisense messages. The genome is rich in transposons, many of which cluster at candidate centromeric regions. The presence of these transposons may drive karyotype instability and phenotypic variation. C. neoformans encodes unique genes that may contribute to its unusual virulence properties, and comparison of two phenotypically distinct strains reveals variation in gene content in addition to sequence polymorphisms between the genomes.

Original language	English
Pages (from-to)	1321-1324
Number of pages	4
Journal	Science
Volume	307
Issue number	5713
DOIs	https://doi.org/10.1126/science.1103773
State	Published - Feb 25 2005

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Loftus, B. J., Fung, E., Roncaglia, P., Rowley, D., Amedeo, P., Bruno, D., Vamathevan, J., Miranda, M., Anderson, I. J., Fraser, J. A., Allen, J. E., Bosdet, I. E., Brent, M. R., Chiu, R., Doering, T. L., Donlin, M. J., D'Souza, C. A., Fox, D. S., Grinberg, V., ... Hyman, R. W. (2005). The genome of the basidiomycetous yeast and human pathogen Cryptococcus neoformans. Science, 307(5713), 1321-1324. https://doi.org/10.1126/science.1103773

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title = "The genome of the basidiomycetous yeast and human pathogen Cryptococcus neoformans",

abstract = "Cryptococcus neoformans is a basidiomycetous yeast ubiquitous in the environment, a model for fungal pathogenesis, and an opportunistic human pathogen of global importance. We have sequenced its ∼20-megabase genome, which contains ∼6500 intron-rich gene structures and encodes a transcriptome abundant in alternatively spliced and antisense messages. The genome is rich in transposons, many of which cluster at candidate centromeric regions. The presence of these transposons may drive karyotype instability and phenotypic variation. C. neoformans encodes unique genes that may contribute to its unusual virulence properties, and comparison of two phenotypically distinct strains reveals variation in gene content in addition to sequence polymorphisms between the genomes.",

author = "Loftus, {Brendan J.} and Eula Fung and Paola Roncaglia and Don Rowley and Paolo Amedeo and Dan Bruno and Jessica Vamathevan and Molly Miranda and Anderson, {Iain J.} and Fraser, {James A.} and Allen, {Jonathan E.} and Bosdet, {Ian E.} and Brent, {Michael R.} and Readman Chiu and Doering, {Tamara L.} and Donlin, {Maureen J.} and D'Souza, {Cletus A.} and Fox, {Deborah S.} and Viktoriya Grinberg and Jianmin Fu and Marilyn Fukushima and Haas, {Brian J.} and Huang, {James C.} and Guilhem Janbon and Jones, {Steven J.M.} and Koo, {Hean L.} and Krzywinski, {Martin I.} and Kwon-Chung, {June K.} and Lengeler, {Klaus B.} and Rama Maiti and Marra, {Marco A.} and Marra, {Robert E.} and Mathewson, {Carrie A.} and Mitchell, {Thomas G.} and Mihaela Pertea and Riggs, {Florenta R.} and Salzberg, {Steven L.} and Schein, {Jacqueline E.} and Alla Shvartsbeyn and Heesun Shin and Martin Shumway and Specht, {Charles A.} and Suh, {Bernard B.} and Aaron Tenney and Utterback, {Terry R.} and Wickes, {Brian L.} and Wortman, {Jennifer R.} and Wye, {Natasja H.} and Kronstad, {James W.} and Lodge, {Jennifer K.} and Joseph Heitman and Davis, {Ronald W.} and Fraser, {Claire M.} and Hyman, {Richard W.}",

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doi = "10.1126/science.1103773",

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Loftus, BJ, Fung, E, Roncaglia, P, Rowley, D, Amedeo, P, Bruno, D, Vamathevan, J, Miranda, M, Anderson, IJ, Fraser, JA, Allen, JE, Bosdet, IE, Brent, MR, Chiu, R, Doering, TL, Donlin, MJ, D'Souza, CA, Fox, DS, Grinberg, V, Fu, J, Fukushima, M, Haas, BJ, Huang, JC, Janbon, G, Jones, SJM, Koo, HL, Krzywinski, MI, Kwon-Chung, JK, Lengeler, KB, Maiti, R, Marra, MA, Marra, RE, Mathewson, CA, Mitchell, TG, Pertea, M, Riggs, FR, Salzberg, SL, Schein, JE, Shvartsbeyn, A, Shin, H, Shumway, M, Specht, CA, Suh, BB, Tenney, A, Utterback, TR, Wickes, BL, Wortman, JR, Wye, NH, Kronstad, JW, Lodge, JK, Heitman, J, Davis, RW, Fraser, CM & Hyman, RW 2005, 'The genome of the basidiomycetous yeast and human pathogen Cryptococcus neoformans', Science, vol. 307, no. 5713, pp. 1321-1324. https://doi.org/10.1126/science.1103773

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T1 - The genome of the basidiomycetous yeast and human pathogen Cryptococcus neoformans

AU - Loftus, Brendan J.

AU - Fung, Eula

AU - Roncaglia, Paola

AU - Rowley, Don

AU - Amedeo, Paolo

AU - Bruno, Dan

AU - Vamathevan, Jessica

AU - Miranda, Molly

AU - Anderson, Iain J.

AU - Fraser, James A.

AU - Allen, Jonathan E.

AU - Bosdet, Ian E.

AU - Brent, Michael R.

AU - Chiu, Readman

AU - Doering, Tamara L.

AU - Donlin, Maureen J.

AU - D'Souza, Cletus A.

AU - Fox, Deborah S.

AU - Grinberg, Viktoriya

AU - Fu, Jianmin

AU - Fukushima, Marilyn

AU - Haas, Brian J.

AU - Huang, James C.

AU - Janbon, Guilhem

AU - Jones, Steven J.M.

AU - Koo, Hean L.

AU - Krzywinski, Martin I.

AU - Kwon-Chung, June K.

AU - Lengeler, Klaus B.

AU - Maiti, Rama

AU - Marra, Marco A.

AU - Marra, Robert E.

AU - Mathewson, Carrie A.

AU - Mitchell, Thomas G.

AU - Pertea, Mihaela

AU - Riggs, Florenta R.

AU - Salzberg, Steven L.

AU - Schein, Jacqueline E.

AU - Shvartsbeyn, Alla

AU - Shin, Heesun

AU - Shumway, Martin

AU - Specht, Charles A.

AU - Suh, Bernard B.

AU - Tenney, Aaron

AU - Utterback, Terry R.

AU - Wickes, Brian L.

AU - Wortman, Jennifer R.

AU - Wye, Natasja H.

AU - Kronstad, James W.

AU - Lodge, Jennifer K.

AU - Heitman, Joseph

AU - Davis, Ronald W.

AU - Fraser, Claire M.

AU - Hyman, Richard W.

PY - 2005/2/25

Y1 - 2005/2/25

N2 - Cryptococcus neoformans is a basidiomycetous yeast ubiquitous in the environment, a model for fungal pathogenesis, and an opportunistic human pathogen of global importance. We have sequenced its ∼20-megabase genome, which contains ∼6500 intron-rich gene structures and encodes a transcriptome abundant in alternatively spliced and antisense messages. The genome is rich in transposons, many of which cluster at candidate centromeric regions. The presence of these transposons may drive karyotype instability and phenotypic variation. C. neoformans encodes unique genes that may contribute to its unusual virulence properties, and comparison of two phenotypically distinct strains reveals variation in gene content in addition to sequence polymorphisms between the genomes.

AB - Cryptococcus neoformans is a basidiomycetous yeast ubiquitous in the environment, a model for fungal pathogenesis, and an opportunistic human pathogen of global importance. We have sequenced its ∼20-megabase genome, which contains ∼6500 intron-rich gene structures and encodes a transcriptome abundant in alternatively spliced and antisense messages. The genome is rich in transposons, many of which cluster at candidate centromeric regions. The presence of these transposons may drive karyotype instability and phenotypic variation. C. neoformans encodes unique genes that may contribute to its unusual virulence properties, and comparison of two phenotypically distinct strains reveals variation in gene content in addition to sequence polymorphisms between the genomes.

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U2 - 10.1126/science.1103773

DO - 10.1126/science.1103773

M3 - Article

C2 - 15653466

AN - SCOPUS:20044380139

SN - 0036-8075

VL - 307

SP - 1321

EP - 1324

JO - Science

JF - Science

IS - 5713

ER -

The genome of the basidiomycetous yeast and human pathogen Cryptococcus neoformans

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