TY - JOUR
T1 - The Fuzzy Logic of Network Connectivity in Mouse Visual Thalamus
AU - Morgan, Josh Lyskowski
AU - Berger, Daniel Raimund
AU - Wetzel, Arthur Willis
AU - Lichtman, Jeff William
N1 - Funding Information:
We gratefully acknowledge support from the NIH/NINDS (1DP2OD006514-01, TR01 1R01NS076467-01, and 1U01NS090449-01), IARPA via DoI/IBC (D16PC00002), Conte (1P50MH094271-01), MURI Army Research Office (contract no. W911NF1210594 and IIS-1447786), NSF (OIA-1125087 and IIS-1110955), the Human Frontier Science Program (RGP0051/2014), and the NIH and NIGMS via the National Center for Multiscale Modeling of Biological Systems (P41GM10371). We are grateful to Linda Xu, Jacob Wasag, Aidan Piper, Seaun Yoo, Shana Attar, Katherine Mateos, Abigail Orlando, Anne Crosby, Kyla Cordrey, Allan Ordonez, and Marquise Bartley, who helped segment the data. We are grateful to James Cuff and his expert staff at Harvard Research Computing for data management, to Hanspeter Pfister and his lab members for help with image storage and alignment, and to Richard Schalek for management and troubleshooting of the EM hardware. We are also grateful to R. Clay Reid, who was involved in the initial planning and support.
Publisher Copyright:
© 2016 Elsevier Inc.
PY - 2016/3/24
Y1 - 2016/3/24
N2 - Summary In an attempt to chart parallel sensory streams passing through the visual thalamus, we acquired a 100-trillion-voxel electron microscopy (EM) dataset and identified cohorts of retinal ganglion cell axons (RGCs) that innervated each of a diverse group of postsynaptic thalamocortical neurons (TCs). Tracing branches of these axons revealed the set of TCs innervated by each RGC cohort. Instead of finding separate sensory pathways, we found a single large network that could not be easily subdivided because individual RGCs innervated different kinds of TCs and different kinds of RGCs co-innervated individual TCs. We did find conspicuous network subdivisions organized on the basis of dendritic rather than neuronal properties. This work argues that, in the thalamus, neural circuits are not based on a canonical set of connections between intrinsically different neuronal types but, rather, may arise by experience-based mixing of different kinds of inputs onto individual postsynaptic cells.
AB - Summary In an attempt to chart parallel sensory streams passing through the visual thalamus, we acquired a 100-trillion-voxel electron microscopy (EM) dataset and identified cohorts of retinal ganglion cell axons (RGCs) that innervated each of a diverse group of postsynaptic thalamocortical neurons (TCs). Tracing branches of these axons revealed the set of TCs innervated by each RGC cohort. Instead of finding separate sensory pathways, we found a single large network that could not be easily subdivided because individual RGCs innervated different kinds of TCs and different kinds of RGCs co-innervated individual TCs. We did find conspicuous network subdivisions organized on the basis of dendritic rather than neuronal properties. This work argues that, in the thalamus, neural circuits are not based on a canonical set of connections between intrinsically different neuronal types but, rather, may arise by experience-based mixing of different kinds of inputs onto individual postsynaptic cells.
UR - http://www.scopus.com/inward/record.url?scp=84961613299&partnerID=8YFLogxK
U2 - 10.1016/j.cell.2016.02.033
DO - 10.1016/j.cell.2016.02.033
M3 - Article
C2 - 27015312
AN - SCOPUS:84961613299
SN - 0092-8674
VL - 165
SP - 192
EP - 206
JO - Cell
JF - Cell
IS - 1
ER -