The flt-1 promoter for transcriptional targeting of teratocarcinoma

Gerd J. Bauerschmitz, Dirk M. Nettelbeck, Anna Kanerva, Andrew H. Baker, Akseli Hemminki, Paul N. Reynolds, David T. Curiel

Research output: Contribution to journalArticlepeer-review

19 Scopus citations

Abstract

Flt-1, a receptor for vascular endothelial growth factor, is known to display dysregulated expression in both tumor vasculature and tumor cells per se, suggesting that the flt-1 promoter might be a useful candidate to achieve tumor-specific transgene expression. In addition, adenoviral vectors containing transgenes under the control of the flt-1 promoter achieve very low levels of expression in the normal liver, the major organ responsible for blood clearance of adenoviruses and inadvertent transgene-related toxicity. Thus, we assessed the ability of adenoviral vectors containing the flt-1 promoter to achieve transgene expression in a range of gynecological and breast tumor lines. High transgene expression levels were detected in teratocarcinoma lines, correlating with levels of flt-1 mRNA. These results suggest that the flt-1 promoter could be useful for transcriptionally targeted gene expression to teratocarcinoma, and that evaluation in other flt-1-positive tumors is warranted.

Original languageEnglish
Pages (from-to)1271-1274
Number of pages4
JournalCancer research
Volume62
Issue number5
StatePublished - Mar 1 2002

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