The expression of Ia antigenic determinants on macrophages required for the in vitro antibody response

J. E. Neiderhuber, P. Allen, L. Mayo

Research output: Contribution to journalArticlepeer-review

34 Scopus citations

Abstract

A subpopulation of antigen-presenting macrophages required for an in vitro antibody response to burro erythrocytes was deleted by pretreating the splenic macrophages with anti-Ia serum and complement (C). The in vitro response of the macrophage depleted T-B cell population could not be restored by the addition of macrophages resistant to anti-Ia antibodies and C (Ia-). The response of Ia- macrophages and the macrophage-depleted T-B cells was only reconstituted by the addition of Ia+ macrophages. Macrophages pretreated with anti-Ia antibodies restricted to react with determinants of one I subregion could not support the in vitro antibody response when added to cultures whose macrophages were pretreated with anti-Ia serum and C specific for the I-J subregion. These results confirmed that Ia determinants of the I-A, the I-E, and the I-C subregions were all expressed on the I-J+ macrophage required for an in vitro antibody response.

Original languageEnglish
Pages (from-to)1342-1349
Number of pages8
JournalJournal of Immunology
Volume122
Issue number4
StatePublished - 1979

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