The expression of GLUT8, GLUT9a, and GLUT9b in the mouse testis and sperm

Tae Kim Sung, Kelle H. Moley

Research output: Contribution to journalArticlepeer-review

54 Scopus citations

Abstract

The objective of this study is to confirm the precise glucose transporter (GLUT) 8 localization and determine the expression of GLUT9a and GLUT9b by Western blot and confocal and immunoelectron microscopy in the mouse testis and sperm. GLUT8, GLUT9a, and GLUT9b proteins are expressed in the most intraseminiferous tubula cells and Leydig cells. GLUT8 localizes in the midpiece and principal piece as well as in the acrosomal region of the sperm. Immunoelectron microscopic analysis shows that GLUT8 is strongly detectable at the acrosome and neck region of the sperm. In the midpiece, GLUT8 localizes at the outer dense fibers (odf) as well as at the circumference of the spiral mitochondria. In the principal piece, GLUT8 localizes at the odf. GLUT9a strictly localizes in the midpiece, but GLUT9b localizes in the acrosome, midpiece, and principal piece of the sperm. These results suggest that glucose uptake via GLUT8, GLUT9a, and GLUT9b likely affects normal spermatogenesis, steroidogenesis, and sperm function in the mouse.

Original languageEnglish
Pages (from-to)445-455
Number of pages11
JournalReproductive Sciences
Volume14
Issue number5
DOIs
StatePublished - Jul 2007

Keywords

  • GLUT8
  • GLUT9a
  • GLUT9b
  • Sperm
  • Testis

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