The expression and function of a group VIA calcium-independent phospholipase A2 (iPLA2β) in β-cells

John Turk, Sasanka Ramanadham

Research output: Contribution to journalArticlepeer-review

31 Scopus citations

Abstract

Many cells express a Group VIA phospholipase A2, designated iPLA2β, that does not require calcium for activation, is stimulated by ATP, and is sensitive to inhibition by a bromoenol lactone suicide substrate (BEL). Studies in various cell systems have led to the suggestion that iPLA2β has a role in phospholipid remodeling, signal transduction, cell proliferation, and apoptosis. We have found that pancreatic islets, β-cells, and glucose-responsive insulinoma cells express an iPLA2β that participates in glucose-stimulated insulin secretion but is not involved in membrane phospholipid remodeling. Additionally, recent studies reveal that iPLA2β is involved in pathways that contribute to β-cell proliferation and apoptosis, and that various phospholipid-derived mediators are involved in these processes. Detailed characterization of the enzyme suggests that the β-cells express multiple isoforms of iPLA2β, and we hypothesize that these participate in different cellular functions.

Original languageEnglish
Pages (from-to)824-832
Number of pages9
JournalCanadian Journal of Physiology and Pharmacology
Volume82
Issue number10
DOIs
StatePublished - Oct 2004

Keywords

  • Apoptosis
  • Isoforms
  • Mass spectrometry
  • Signalling

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