TY - JOUR
T1 - The epithelial potassium channel kir7.1 is stimulated by progesterone
AU - Björkgren, Ida
AU - Mendoza, Sarah
AU - Chung, Dong Hwa
AU - Haoui, Monika
AU - Petersen, Natalie True
AU - Lishko, Polina V.
N1 - Funding Information:
This work was supported by National Institutes of Health grant R01GM111802, a Pew Innovation Fund 2020 award from Pew Charitable Trusts, a Rose Hills Foundation award, and Packer Wentz Endowment Will. This work was also funded in part by the Global Consortium for Reproductive Longevity and Equality at the Buck Institute, made possible by the Bia-Echo Foundation, award GCRLE-0920 (P.V. Lishko). This work also used the Vincent J. Coates Genomics Sequencing Laboratory at University of California, Berkeley, which is supported by National Institutes of Health instrumentation grant S10 OD018174.
Publisher Copyright:
© 2021 Björkgren et al.
PY - 2021/10/4
Y1 - 2021/10/4
N2 - The choroid plexus (CP) epithelium secretes cerebrospinal fluid and plays an important role in healthy homeostasis of the brain. CP function can be influenced by sex steroid hormones; however, the precise molecular mechanism of such regulation is not well understood. Here, using whole-cell patch-clamp recordings from male and female murine CP cells, we show that application of progesterone resulted in specific and strong potentiation of the inwardly rectifying potassium channel Kir7.1, an essential protein that is expressed in CP and is required for survival. The potentiation was progesterone specific and independent of other known progesterone receptors expressed in CP. This effect was recapitulated with recombinant Kir7.1, as well as with endogenous Kir7.1 expressed in the retinal pigment epithelium. Current-clamp studies further showed a progesterone-induced hyperpolarization of CP cells. Our results provide evidence of a progesterone-driven control of tissues in which Kir7.1 is present.
AB - The choroid plexus (CP) epithelium secretes cerebrospinal fluid and plays an important role in healthy homeostasis of the brain. CP function can be influenced by sex steroid hormones; however, the precise molecular mechanism of such regulation is not well understood. Here, using whole-cell patch-clamp recordings from male and female murine CP cells, we show that application of progesterone resulted in specific and strong potentiation of the inwardly rectifying potassium channel Kir7.1, an essential protein that is expressed in CP and is required for survival. The potentiation was progesterone specific and independent of other known progesterone receptors expressed in CP. This effect was recapitulated with recombinant Kir7.1, as well as with endogenous Kir7.1 expressed in the retinal pigment epithelium. Current-clamp studies further showed a progesterone-induced hyperpolarization of CP cells. Our results provide evidence of a progesterone-driven control of tissues in which Kir7.1 is present.
UR - http://www.scopus.com/inward/record.url?scp=85114385364&partnerID=8YFLogxK
U2 - 10.1085/jgp.202112924
DO - 10.1085/jgp.202112924
M3 - Article
C2 - 34387656
AN - SCOPUS:85114385364
SN - 0022-1295
VL - 153
JO - Journal of General Physiology
JF - Journal of General Physiology
IS - 10
M1 - e202112924
ER -