The ENTPD1 promoter polymorphism -860 A > G (rs3814159) is associated with increased gene transcription, protein expression, CD39/NTPDase1 enzymatic activity, and thromboembolism risk

James P. Maloney, Brian R. Branchford, Gary L. Brodsky, Maxwell S. Cosmic, David W. Calabrese, Christina L. Aquilante, Kelly W. Maloney, Joseph R. Gonzalez, Weiming Zhang, Kerrie L. Moreau, Kerri L. Wiggins, Nicholas L. Smith, Ulrich Broeckel, Jorge Di Paola

Research output: Contribution to journalArticle

4 Scopus citations

Abstract

Ectonucleoside triphosphate diphosphohydrolase 1 (NTPDase1) degrades the purines ATP and ADP that are key regulators of inflammation and clotting. We hypothesized that NTPDase1 polymorphisms exist and that they regulate this pathway. We sequenced the ENTPD1 gene (encoding NTPDase1) in 216 subjects then assessed genotypes in 2 cohorts comprising 2213 humans to identify ENTPD1 polymorphisms associated with venous thromboembolism (VTE). The G allele of the intron 1 polymorphism rs3176891 was more common in VTE vs. controls (odds ratio 1.26–1.9); it did not affect RNA splicing, but it was in strong linkage disequilibrium with the G allele of the promoter polymorphism rs3814159, which increased transcriptional activity by 8-fold. Oligonucleotides containing the G allele of this promoter region bound nuclear extracts more avidly. Carriers of rs3176891 G had endothelial cells with increased NTPDase1 activity and protein expression, and had platelets with enhanced aggregation. Thus, the G allele of rs3176891 marks a haplotype associated with increased clotting and platelet aggregation attributable to a promoter variant associated with increased transcription, expression, and activity of NTPDase1. We term this gain-of-function phenotype observed with rs3814159 G “CD39 Denver.”

Original languageEnglish
Pages (from-to)2771-2784
Number of pages14
JournalFASEB Journal
Volume31
Issue number7
DOIs
StatePublished - Jul 2017
Externally publishedYes

Keywords

  • CD39 Denver
  • P2Y1
  • Platelet aggregation
  • Purinergic signaling

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    Maloney, J. P., Branchford, B. R., Brodsky, G. L., Cosmic, M. S., Calabrese, D. W., Aquilante, C. L., Maloney, K. W., Gonzalez, J. R., Zhang, W., Moreau, K. L., Wiggins, K. L., Smith, N. L., Broeckel, U., & Di Paola, J. (2017). The ENTPD1 promoter polymorphism -860 A > G (rs3814159) is associated with increased gene transcription, protein expression, CD39/NTPDase1 enzymatic activity, and thromboembolism risk. FASEB Journal, 31(7), 2771-2784. https://doi.org/10.1096/fj.201600344R