The effects of storage time and sampling season on the stability of serum 25-hydroxy vitamin D and androstenedione

Calypse Agborsangaya, Adetunji T. Toriola, Kjell Grankvist, Heljia Marja Surcel, Katsiaryna Holl, Seppo Parkkila, Pentti Tuohimaa, Annekatrin Lukanova, Matti Lehtinen

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Abstract

Knowledge of the stability of serum samples stored in large biobanks is pivotal for reliable assessment of hormone-dependent disease risks. We studied the effects of sample storage time and season of serum sampling on the stability of 25-hydroxy vitamin D (25-OHD) and androstenedione in a stratified random sample of 402 women, using paired sera from the Finnish Maternity Cohort. Serum samples selected were donated between 6 and 24 yr ago. The storage time did not affect serum 25-OHD and androstenedione levels. However, there was a significant mean difference in the 25-OHD levels of sera withdrawn during winter (first sample) vs. during summer (second sample; -18.4 nmol/l, P 0.001). Also at the individual level, there were significant differences in average 25-OHD levels between individuals with the paired sera taken at winter-winter compared with other alternatives (summer-winter, winter-summer, and summer-summer). The androstenedione levels showed no such differences. Long-term storage does not affect serum 25-OHD and androstenedione levels, but sampling season is an important determinant of 25-OHD levels. Stored serum samples can be used to study disease associations with both hormones. However, sampling season needs to be taken into account for 25-OHD by considering matching and stratification and, if possible, serial sampling.

Original languageEnglish
Pages (from-to)51-57
Number of pages7
JournalNutrition and Cancer
Volume62
Issue number1
DOIs
StatePublished - Jan 1 2010
Externally publishedYes

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    Agborsangaya, C., Toriola, A. T., Grankvist, K., Surcel, H. M., Holl, K., Parkkila, S., Tuohimaa, P., Lukanova, A., & Lehtinen, M. (2010). The effects of storage time and sampling season on the stability of serum 25-hydroxy vitamin D and androstenedione. Nutrition and Cancer, 62(1), 51-57. https://doi.org/10.1080/01635580903191460