TY - JOUR
T1 - The effects of elastic fiber protein insufficiency and treatment on the modulus of arterial smooth muscle cells
AU - Espinosa, M. Gabriela
AU - Gardner, William S.
AU - Bennett, Lisa
AU - Sather, Bradley A.
AU - Yanagisawa, Hiromi
AU - Wagenseil, Jessica E.
PY - 2014/2
Y1 - 2014/2
N2 - Elastic fibers are critical for the mechanical function of the large arteries. Mechanical effects of elastic fiber protein deficiency have been investigated in whole arteries, but not in isolated smooth muscle cells (SMCs). The elastic moduli of SMCs from elastin (Eln-/-) and fibulin-4 (Fbln4-/-) knockout mice were measured using atomic force microscopy. Compared to control SMCs, the modulus of Eln-/-SMCs is reduced by 40%, but is unchanged in Fbln4-/-SMCs. The Eln-/-SMC modulus is rescued by soluble or α elastin treatment. Altered gene expression, specifically of calponin, suggests that SMC phenotypic modulation may be responsible for the modulus changes.
AB - Elastic fibers are critical for the mechanical function of the large arteries. Mechanical effects of elastic fiber protein deficiency have been investigated in whole arteries, but not in isolated smooth muscle cells (SMCs). The elastic moduli of SMCs from elastin (Eln-/-) and fibulin-4 (Fbln4-/-) knockout mice were measured using atomic force microscopy. Compared to control SMCs, the modulus of Eln-/-SMCs is reduced by 40%, but is unchanged in Fbln4-/-SMCs. The Eln-/-SMC modulus is rescued by soluble or α elastin treatment. Altered gene expression, specifically of calponin, suggests that SMC phenotypic modulation may be responsible for the modulus changes.
UR - http://www.scopus.com/inward/record.url?scp=84896793085&partnerID=8YFLogxK
U2 - 10.1115/1.4026203
DO - 10.1115/1.4026203
M3 - Article
C2 - 24322348
AN - SCOPUS:84896793085
SN - 0148-0731
VL - 136
JO - Journal of Biomechanical Engineering
JF - Journal of Biomechanical Engineering
IS - 2
M1 - 021030
ER -