TY - JOUR
T1 - The effects of a diet rich in fish oil on human neutrophils
T2 - Identification of leukotriene B5 as a metabolite
AU - Prescott, Stephen M.
AU - Zimmerman, Guy A.
AU - Morrison, Aubrey R.
N1 - Funding Information:
We thank Connie Schipke, of the University of Utah Clinical Research Center, for designing and supervising the experimental diet. We are grateful to Anthony R. Seeger and Deborah Kline-Knoeckel for technical assistance, and to Carol Evans and Leona Archuleta for preparing the illustrations and manuscript. Drs. Ray Rice and Stuart Reed generously supplied the MaxEPA. We thank Dr. Joshua Rokach (Merck-Frosst, Montreal) for authentic standards of LTB~. This work was supported by grants from the Nora Eccles Treadwell Foundation, the Montana Heart Association, and Public Health Service Research Grant #RR-64 from the Division of Research Resources. Dr. Zimmerman is the recipient of Public Health Service Clinical Investigator Award (#HL00696) from the National Heart, Lung, and Blood Institute. Dr. Morrison is an Established Investigator of the American Heart Association.
PY - 1985/8
Y1 - 1985/8
N2 - Diets that are enriched with fish oil have been shown to alter arachidonic acid metabolism via the cyclooxygenase pathway. Recently it has been shown that one of the major component fatty acids of fish oil, eicosapentaenoate (EPA), is a substrate for the leukotriene B (LTB) pathway when added exogenously to human neutrophils in vitro. We fed a diet that contained 8-10 gm/day of EPA to four human subjects for three weeks and compared the arachidonate metabolism of their neutrophils to the same functions while the subjects were on their usual diet. The fish oil-supplementation increased neutrophil EPA content from undetectable levels to 7.4 ± 2.4% (p<0.01, expressed as % of total fatty acid), and decreased arachidonate from 15.4 ± 2.3% to 12.8 ± 2.3% (p<0.05). Leukotriene B5 was identified as a metabolite during the fish oil-diet by its chromatographic profile and mass spectrum. During the experimental diet LTB4 decreased from 160 ± 37 ng/107 neutrophils to 120 ± 12 (p<0.05), and LTB5 increased from 0 to 39 ± 9 ng/107 neutrophils (p<0.005). The diet had no effect on neutrophil aggregation or adherence to nylon fibers.
AB - Diets that are enriched with fish oil have been shown to alter arachidonic acid metabolism via the cyclooxygenase pathway. Recently it has been shown that one of the major component fatty acids of fish oil, eicosapentaenoate (EPA), is a substrate for the leukotriene B (LTB) pathway when added exogenously to human neutrophils in vitro. We fed a diet that contained 8-10 gm/day of EPA to four human subjects for three weeks and compared the arachidonate metabolism of their neutrophils to the same functions while the subjects were on their usual diet. The fish oil-supplementation increased neutrophil EPA content from undetectable levels to 7.4 ± 2.4% (p<0.01, expressed as % of total fatty acid), and decreased arachidonate from 15.4 ± 2.3% to 12.8 ± 2.3% (p<0.05). Leukotriene B5 was identified as a metabolite during the fish oil-diet by its chromatographic profile and mass spectrum. During the experimental diet LTB4 decreased from 160 ± 37 ng/107 neutrophils to 120 ± 12 (p<0.05), and LTB5 increased from 0 to 39 ± 9 ng/107 neutrophils (p<0.005). The diet had no effect on neutrophil aggregation or adherence to nylon fibers.
UR - http://www.scopus.com/inward/record.url?scp=0022380094&partnerID=8YFLogxK
U2 - 10.1016/0090-6980(85)90186-8
DO - 10.1016/0090-6980(85)90186-8
M3 - Article
C2 - 2996057
AN - SCOPUS:0022380094
SN - 0090-6980
VL - 30
SP - 209
EP - 227
JO - Prostaglandins
JF - Prostaglandins
IS - 2
ER -