TY - JOUR
T1 - The effect on synaptic physiology of synaptotagmin mutations in drosophila
AU - DiAntonio, Aaron
AU - Schwarz, Thomas L.
N1 - Funding Information:
We wish to thank Karen Parfitt and Felix Schweizer for advice about the physiology experiments, Matthew Fienstein for help with the image analysis, and Troy Littleton and Hugo Bellen for fly stocks and antiserum. We also wish to thank Irene lnman for excellent technical assistance and Robert Burgess, David Deitcher, Andrew Randall, Noreen Reist, Tim Ryan, and Felix Schweizer for discussion and for comments on this manuscript. This work was supported by the McKnight Foundation and a Silvio Conte Center for Neuroscience Award from the National institute of Mental Health. A. D. was supported by a Medical Scientist Training Program fellowship from the National Institute of General Medical Sciences (5T32 GM07365). The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be
PY - 1994/4
Y1 - 1994/4
N2 - Synaptotagmin is a synaptic vesicle protein implicated in neurotransmitter release. Molecular characterization of four mutant alleles of this protein in Drosophila melanogaster has permitted an investigation of synaptotagmin's role in synaptic physiology and of some of the structural requirements for its function. Reduced levels of synaptotagmin resulted in a substantial alteration in synaptic function in the eye and at larval neuromuscular junctions. Decreased neurotransmitter release caused smaller evoked synaptic potentials. However, the frequency, but not the size, of spontaneous quantal events was simultaneously increased. These abnormalities do not appear to be secondary to a detectable morphological change in the arborization of the synapse. The increased frequency of spontaneous events was insufficient to deplete significantly the vesicle supply and thereby account for reduced transmission. These data are discussed in the context of models in which synaptotagmin's function includes a role in vesicle docking.
AB - Synaptotagmin is a synaptic vesicle protein implicated in neurotransmitter release. Molecular characterization of four mutant alleles of this protein in Drosophila melanogaster has permitted an investigation of synaptotagmin's role in synaptic physiology and of some of the structural requirements for its function. Reduced levels of synaptotagmin resulted in a substantial alteration in synaptic function in the eye and at larval neuromuscular junctions. Decreased neurotransmitter release caused smaller evoked synaptic potentials. However, the frequency, but not the size, of spontaneous quantal events was simultaneously increased. These abnormalities do not appear to be secondary to a detectable morphological change in the arborization of the synapse. The increased frequency of spontaneous events was insufficient to deplete significantly the vesicle supply and thereby account for reduced transmission. These data are discussed in the context of models in which synaptotagmin's function includes a role in vesicle docking.
UR - https://www.scopus.com/pages/publications/0028351171
U2 - 10.1016/0896-6273(94)90342-5
DO - 10.1016/0896-6273(94)90342-5
M3 - Article
C2 - 7909234
AN - SCOPUS:0028351171
SN - 0896-6273
VL - 12
SP - 909
EP - 920
JO - Neuron
JF - Neuron
IS - 4
ER -