TY - JOUR
T1 - The effect of specimen processing on radiolabeled monoclonal antibody biodistribution
AU - Wahl, Richard L.
AU - Sherman, Philip
AU - Fisher, Susan
N1 - Copyright:
Copyright 2007 Elsevier B.V., All rights reserved.
PY - 1984/8
Y1 - 1984/8
N2 - Monoclonal antibodies are assuming increasing importance in experimental and clinical medicine. Generally, tissue biodistribution studies in animals precede human studies. To investigate a concern of ours that varying methods of sample handling in these studies could result in apparent alterations in tissue-binding levels, we compared two methods of tissue processing after the administration of labeled antibodies: one including only blotting away of blood, the other involving several washing steps. The unwashed, blotted specimens were found to have significantly more radioactivity per gram of tissue than the washed, ranging from 22% more in the spleen to 52% more in the lungs and left ventricle. Since in vivo imaging is dependent on the total mount of radioactivity in an organ, we believe the most meaningful determination of tissue radioactivity should be based on unwashed samples. Awareness of this problem is suggested to allow meaningful extrapolations from measured tisue localization data to imaging and therapy.
AB - Monoclonal antibodies are assuming increasing importance in experimental and clinical medicine. Generally, tissue biodistribution studies in animals precede human studies. To investigate a concern of ours that varying methods of sample handling in these studies could result in apparent alterations in tissue-binding levels, we compared two methods of tissue processing after the administration of labeled antibodies: one including only blotting away of blood, the other involving several washing steps. The unwashed, blotted specimens were found to have significantly more radioactivity per gram of tissue than the washed, ranging from 22% more in the spleen to 52% more in the lungs and left ventricle. Since in vivo imaging is dependent on the total mount of radioactivity in an organ, we believe the most meaningful determination of tissue radioactivity should be based on unwashed samples. Awareness of this problem is suggested to allow meaningful extrapolations from measured tisue localization data to imaging and therapy.
UR - http://www.scopus.com/inward/record.url?scp=0021125528&partnerID=8YFLogxK
U2 - 10.1007/BF00252876
DO - 10.1007/BF00252876
M3 - Article
C2 - 6489371
AN - SCOPUS:0021125528
VL - 9
SP - 382
EP - 384
JO - European Journal of Nuclear Medicine
JF - European Journal of Nuclear Medicine
SN - 0340-6997
IS - 8
ER -