TY - JOUR
T1 - The Effect of RGD Peptide on 2D and Miniaturized 3D Culture of HEPM Cells, MSCs, and ADSCs with Alginate Hydrogel
AU - Dumbleton, Jenna
AU - Agarwal, Pranay
AU - Huang, Haishui
AU - Hogrebe, Nathaniel
AU - Han, Renzhi
AU - Gooch, Keith J.
AU - He, Xiaoming
N1 - Funding Information:
This work was partially supported by grants from NSF (CBET-1033426) and NIH (R01EB012108). KJG was supported by grant (CMMI-1334757) from NSF. RZ was supported by grants (R01HL116546 and R01AR064241) from NIH. We would like to thank Dr. Lisa Hommel in The Ohio State University Surface Analysis Facility for her assistance in conducting and analyzing the XPS spectra and Anne Shim for her technical help.
Publisher Copyright:
© 2016, Biomedical Engineering Society.
PY - 2016/6/1
Y1 - 2016/6/1
N2 - Advancements in tissue engineering require the development of new technologies to study cell behavior in vitro. This study focuses on stem cell behavior within various miniaturized three-dimensional (3D) culture conditions of alginate biomaterials modified with the Arg-Gly-Asp (RGD) peptide known for its role in cell adhesion/attachment. Human embryonic palatal mesenchyme (HEPM) cells, bone marrow derived mesenchymal stem cells (MSCs), and human adipose derived stem cells (ADSCs) were cultured on a flat hydrogel of different concentrations of alginate-RGD, and in the miniaturized 3D core of microcapsules with either a 2% alginate or 2% alginate-RGD shell. The core was made of 0, 0.5, or 2% alginate-RGD. Cell spreading was observed in all systems containing the RGD peptide, and the cell morphology was quantified by measuring the cell surface area and circularity. For all types of stem cells, there was a significant increase in the cell surface area (p < 0.05) and a significant decrease in cell circularity (p < 0.01) in alginate-RGD conditions, indicating that cells spread much more readily in environments containing the peptide. This control over the cell spreading within a 3D microenvironment can help to create the ideal biomimetic condition for conducting further studies on cell behavior.
AB - Advancements in tissue engineering require the development of new technologies to study cell behavior in vitro. This study focuses on stem cell behavior within various miniaturized three-dimensional (3D) culture conditions of alginate biomaterials modified with the Arg-Gly-Asp (RGD) peptide known for its role in cell adhesion/attachment. Human embryonic palatal mesenchyme (HEPM) cells, bone marrow derived mesenchymal stem cells (MSCs), and human adipose derived stem cells (ADSCs) were cultured on a flat hydrogel of different concentrations of alginate-RGD, and in the miniaturized 3D core of microcapsules with either a 2% alginate or 2% alginate-RGD shell. The core was made of 0, 0.5, or 2% alginate-RGD. Cell spreading was observed in all systems containing the RGD peptide, and the cell morphology was quantified by measuring the cell surface area and circularity. For all types of stem cells, there was a significant increase in the cell surface area (p < 0.05) and a significant decrease in cell circularity (p < 0.01) in alginate-RGD conditions, indicating that cells spread much more readily in environments containing the peptide. This control over the cell spreading within a 3D microenvironment can help to create the ideal biomimetic condition for conducting further studies on cell behavior.
KW - 3D culture
KW - Biomimetic
KW - Core–shell microcapsules
KW - Stem cell
UR - http://www.scopus.com/inward/record.url?scp=84955325260&partnerID=8YFLogxK
U2 - 10.1007/s12195-016-0428-9
DO - 10.1007/s12195-016-0428-9
M3 - Article
AN - SCOPUS:84955325260
SN - 1865-5025
VL - 9
SP - 277
EP - 288
JO - Cellular and Molecular Bioengineering
JF - Cellular and Molecular Bioengineering
IS - 2
ER -