The Effect of RGD Peptide on 2D and Miniaturized 3D Culture of HEPM Cells, MSCs, and ADSCs with Alginate Hydrogel

Jenna Dumbleton, Pranay Agarwal, Haishui Huang, Nathaniel Hogrebe, Renzhi Han, Keith J. Gooch, Xiaoming He

Research output: Contribution to journalArticlepeer-review

28 Scopus citations

Abstract

Advancements in tissue engineering require the development of new technologies to study cell behavior in vitro. This study focuses on stem cell behavior within various miniaturized three-dimensional (3D) culture conditions of alginate biomaterials modified with the Arg-Gly-Asp (RGD) peptide known for its role in cell adhesion/attachment. Human embryonic palatal mesenchyme (HEPM) cells, bone marrow derived mesenchymal stem cells (MSCs), and human adipose derived stem cells (ADSCs) were cultured on a flat hydrogel of different concentrations of alginate-RGD, and in the miniaturized 3D core of microcapsules with either a 2% alginate or 2% alginate-RGD shell. The core was made of 0, 0.5, or 2% alginate-RGD. Cell spreading was observed in all systems containing the RGD peptide, and the cell morphology was quantified by measuring the cell surface area and circularity. For all types of stem cells, there was a significant increase in the cell surface area (p < 0.05) and a significant decrease in cell circularity (p < 0.01) in alginate-RGD conditions, indicating that cells spread much more readily in environments containing the peptide. This control over the cell spreading within a 3D microenvironment can help to create the ideal biomimetic condition for conducting further studies on cell behavior.

Original languageEnglish
Pages (from-to)277-288
Number of pages12
JournalCellular and Molecular Bioengineering
Volume9
Issue number2
DOIs
StatePublished - Jun 1 2016

Keywords

  • 3D culture
  • Biomimetic
  • Core–shell microcapsules
  • Stem cell

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