The effect of dietary fat intake on hepatic gene expression in LG/J AND SM/J mice

Charlyn G. Partridge, Gloria L. Fawcett, Bing Wang, Clay F. Semenkovich, James M. Cheverud

Research output: Contribution to journalArticlepeer-review

24 Scopus citations

Abstract

Background: The liver plays a major role in regulating metabolic homeostasis and is vital for nutrient metabolism. Identifying the genetic factors regulating these processes could lead to a greater understanding of how liver function responds to a high-fat diet and how that response may influence susceptibilities to obesity and metabolic syndrome. In this study we examine differences in hepatic gene expression between the LG/J and SM/J inbred mouse strains and how gene expression in these strains is affected by high-fat diet. LG/J and SM/J are known to differ in their responses to a high-fat diet for a variety of obesity- and diabetes-related traits, with the SM/J strain exhibiting a stronger phenotypic response to diet. Results: Dietary intake had a significant effect on gene expression in both inbred lines. Genes up-regulated by a high-fat diet were involved in biological processes such as lipid and carbohydrate metabolism; protein and amino acid metabolic processes were down regulated on a high-fat diet. A total of 259 unique transcripts exhibited a significant diet-by-strain interaction. These genes tended to be associated with immune function. In addition, genes involved in biochemical processes related to non-alcoholic fatty liver disease (NAFLD) manifested different responses to diet between the two strains. For most of these genes, SM/J had a stronger response to the high-fat diet than LG/J. Conclusions: These data show that dietary fat impacts gene expression levels in SM/J relative to LG/J, with SM/J exhibiting a stronger response. This supports previous data showing that SM/J has a stronger phenotypic response to high-fat diet. Based upon these findings, we suggest that SM/J and its cross with the LG/J strain provide a good model for examining non-alcoholic fatty liver disease and its role in metabolic syndrome.

Original languageEnglish
Article number99
JournalBMC genomics
Volume15
Issue number1
DOIs
StatePublished - Feb 5 2014

Keywords

  • Dietary fat
  • Gene expression
  • LG/J
  • Liver
  • Microarray
  • NAFLD
  • Non-alcoholic fatty liver disease
  • SM/J

Fingerprint

Dive into the research topics of 'The effect of dietary fat intake on hepatic gene expression in LG/J AND SM/J mice'. Together they form a unique fingerprint.

Cite this