The Duodenal 1α, 25-Dihydroxyvitamin D3 receptor in rats with experimentally induced diabetes

Yoshiki Seino, Rosa I. Sierra, Young Mee Sonn, Aghdass Jafari, Stanley J. Birge, Louis V. Avioli

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18 Scopus citations

Abstract

Studies in the rat with streptozotocin-induced diabetes of short and long duration revealed decreased circulat-ing lα, 25-dihydroxyvitamin D [1, 25-(OH)2D] levels and an in-tact 1, 25-(OH)2D3 duodenal cytosolic receptor with a sedimentation coefficient of 3.3S. Whereas no significant alterations in the equilibrium dissociation constant (Kd) were observed in the diabetic animals, the number of l, 25-(OH)2D3-binding sites was increased in the animals with short term (235 ± 48 us. 100 ± 15 fmol/mg protein) and long term (521 ± 60 vs. 119 ± 15 fmol/mg protein) diabetes. The data are consistent with the hypothesis that alterations in intestinal calcium absorption previously observed in the diabetic state are due, at least in part, to dynamic relationships between circulating 1, 25-(OH)2D concentrations and the number of intestinal l, 25(OH)2D3-binding sites.

Original languageEnglish
Pages (from-to)1721-1725
Number of pages5
JournalEndocrinology
Volume113
Issue number5
DOIs
StatePublished - Nov 1983

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