TY - JOUR
T1 - The Duodenal 1α, 25-Dihydroxyvitamin D3 receptor in rats with experimentally induced diabetes
AU - Seino, Yoshiki
AU - Sierra, Rosa I.
AU - Sonn, Young Mee
AU - Jafari, Aghdass
AU - Birge, Stanley J.
AU - Avioli, Louis V.
PY - 1983/11
Y1 - 1983/11
N2 - Studies in the rat with streptozotocin-induced diabetes of short and long duration revealed decreased circulat-ing lα, 25-dihydroxyvitamin D [1, 25-(OH)2D] levels and an in-tact 1, 25-(OH)2D3 duodenal cytosolic receptor with a sedimentation coefficient of 3.3S. Whereas no significant alterations in the equilibrium dissociation constant (Kd) were observed in the diabetic animals, the number of l, 25-(OH)2D3-binding sites was increased in the animals with short term (235 ± 48 us. 100 ± 15 fmol/mg protein) and long term (521 ± 60 vs. 119 ± 15 fmol/mg protein) diabetes. The data are consistent with the hypothesis that alterations in intestinal calcium absorption previously observed in the diabetic state are due, at least in part, to dynamic relationships between circulating 1, 25-(OH)2D concentrations and the number of intestinal l, 25(OH)2D3-binding sites.
AB - Studies in the rat with streptozotocin-induced diabetes of short and long duration revealed decreased circulat-ing lα, 25-dihydroxyvitamin D [1, 25-(OH)2D] levels and an in-tact 1, 25-(OH)2D3 duodenal cytosolic receptor with a sedimentation coefficient of 3.3S. Whereas no significant alterations in the equilibrium dissociation constant (Kd) were observed in the diabetic animals, the number of l, 25-(OH)2D3-binding sites was increased in the animals with short term (235 ± 48 us. 100 ± 15 fmol/mg protein) and long term (521 ± 60 vs. 119 ± 15 fmol/mg protein) diabetes. The data are consistent with the hypothesis that alterations in intestinal calcium absorption previously observed in the diabetic state are due, at least in part, to dynamic relationships between circulating 1, 25-(OH)2D concentrations and the number of intestinal l, 25(OH)2D3-binding sites.
UR - http://www.scopus.com/inward/record.url?scp=0020565353&partnerID=8YFLogxK
U2 - 10.1210/endo-113-5-1721
DO - 10.1210/endo-113-5-1721
M3 - Article
C2 - 6354695
AN - SCOPUS:0020565353
VL - 113
SP - 1721
EP - 1725
JO - Endocrinology
JF - Endocrinology
SN - 0013-7227
IS - 5
ER -