Abstract
The double-helix point spread function microscope encodes the axial (z) position information of single emitters in wide-field (x,y) images, thus enabling localization in three dimensions (3D) inside extended volumes. We experimentally determine the statistical localization precision σof this approach using single emitters in a cell under typical background conditions, demonstrating σ<20 nm laterally and <30 nm axially for N ≈ 1180 photons per localization. Combined with light-induced blinking of single-molecule labels, we present proof-of-concept imaging beyond the optical diffraction limit of microtubule network structures in fixed mammalian cells over a large axial range in three dimensions.
Original language | English |
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Article number | 153701 |
Journal | Applied Physics Letters |
Volume | 100 |
Issue number | 15 |
DOIs | |
State | Published - Apr 9 2012 |