The direct interaction of NME3 with Tip60 in DNA repair

Ning Tsao, Ya Chi Yang, Yu Jyun Deng, Zee Fen Chang

Research output: Contribution to journalArticlepeer-review

13 Scopus citations

Abstract

Cellular supply of dNTPs via RNR (ribonucleotide reductase) is crucial for DNA replication and repair. It has been shown that DNA-damage-site-specific recruitment of RNR is critical for DNA repair efficiency in quiescent cells. The catalytic function of RNR produces dNDPs. The subsequent step of dNTP formation requires the function of NDP kinase. There are ten isoforms of NDP kinase in human cells. In the present study, we identified NME3 as one specific NDP kinase that interacts directly with Tip60, a histone acetyltransferase, to form a complex with RNR. Our data reveal that NME3 recruitment to DNA damage sites depends on this interaction. Disruption of interaction of NME3 with Tip60 suppressed DNA repair in serum-deprived cells. Thus Tip60 interacts with RNR and NME3 to provide site-specific synthesis of dNTP for facilitating DNA repair in serum-deprived cells which contain low levels of dNTPs.

Original languageEnglish
Pages (from-to)1237-1245
Number of pages9
JournalBiochemical Journal
Volume473
Issue number9
DOIs
StatePublished - May 1 2016

Keywords

  • DNA repair
  • NME3
  • Ribonucleotide reductase
  • Tip60

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