TY - JOUR
T1 - The dimeric architecture of checkpoint kinases Mec1ATR and Tel1ATM reveal a common structural organization
AU - Sawicka, Marta
AU - Wanrooij, Paulina H.
AU - Darbari, Vidya C.
AU - Tannous, Elias
AU - Hailemariam, Sarem
AU - Bose, Daniel
AU - Makarova, Alena V.
AU - Burgers, Peter M.
AU - Zhang, Xiaodong
N1 - Publisher Copyright:
© 2016 by The American Society for Biochemistry and Molecular Biology, Inc.
PY - 2016/6/24
Y1 - 2016/6/24
N2 - The phosphatidylinositol 3-kinase-related protein kinases are key regulators controlling a wide range of cellular events. The yeast Tel1 and Mec1·Ddc2 complex (ATM and ATR-ATRIP in humans) play pivotal roles in DNA replication, DNA damage signaling, and repair. Here, we present the first structural insight for dimers of Mec1·Ddc2 and Tel1 using single-particle electron microscopy. Both kinases reveal a head to head dimer with one major dimeric interface through the N-terminal HEAT (named after Huntingtin, elongation factor 3, protein phosphatase 2A, and yeast kinase TOR1) repeat. Their dimeric interface is significantly distinct from the interface of mTOR complex 1 dimer, which oligomerizes through two spatially separate interfaces. We also observe different structural organizations of kinase domains of Mec1 and Tel1. The kinase domains in the Mec1·Ddc2 dimer are located in close proximity to each other. However, in the Tel1 dimer they are fully separated, providing potential access of substrates to this kinase, even in its dimeric form.
AB - The phosphatidylinositol 3-kinase-related protein kinases are key regulators controlling a wide range of cellular events. The yeast Tel1 and Mec1·Ddc2 complex (ATM and ATR-ATRIP in humans) play pivotal roles in DNA replication, DNA damage signaling, and repair. Here, we present the first structural insight for dimers of Mec1·Ddc2 and Tel1 using single-particle electron microscopy. Both kinases reveal a head to head dimer with one major dimeric interface through the N-terminal HEAT (named after Huntingtin, elongation factor 3, protein phosphatase 2A, and yeast kinase TOR1) repeat. Their dimeric interface is significantly distinct from the interface of mTOR complex 1 dimer, which oligomerizes through two spatially separate interfaces. We also observe different structural organizations of kinase domains of Mec1 and Tel1. The kinase domains in the Mec1·Ddc2 dimer are located in close proximity to each other. However, in the Tel1 dimer they are fully separated, providing potential access of substrates to this kinase, even in its dimeric form.
UR - http://www.scopus.com/inward/record.url?scp=84975887007&partnerID=8YFLogxK
U2 - 10.1074/jbc.M115.708263
DO - 10.1074/jbc.M115.708263
M3 - Article
C2 - 27129217
AN - SCOPUS:84975887007
SN - 0021-9258
VL - 291
SP - 13436
EP - 13447
JO - Journal of Biological Chemistry
JF - Journal of Biological Chemistry
IS - 26
ER -