The differentiation of embryonic stem cells seeded on electrospun nanofibers into neural lineages

Jingwei Xie; Stephanie M. Willerth; Xiaoran Li; Matthew R. Macewan; Allison Rader; Shelly E. Sakiyama-Elbert; Younan Xia

doi:10.1016/j.biomaterials.2008.09.046

The differentiation of embryonic stem cells seeded on electrospun nanofibers into neural lineages

Jingwei Xie
, Stephanie M. Willerth
, Xiaoran Li
, Matthew R. Macewan
, Allison Rader
, Shelly E. Sakiyama-Elbert
, Younan Xia

Research output: Contribution to journal › Article › peer-review

399 Scopus citations

Abstract

Due to advances in stem cell biology, embryonic stem (ES) cells can be induced to differentiate into a particular mature cell lineage when cultured as embryoid bodies. Although transplantation of ES cells-derived neural progenitor cells has been demonstrated with some success for either spinal cord injury repair in small animal model, control of ES cell differentiation into complex, viable, higher ordered tissues is still challenging. Mouse ES cells have been induced to become neural progenitors by adding retinoic acid to embryoid body cultures for 4 days. In this study, we examine the use of electrospun biodegradable polymers as scaffolds not only for enhancing the differentiation of mouse ES cells into neural lineages but also for promoting and guiding the neurite outgrowth. A combination of electrospun fiber scaffolds and ES cells-derived neural progenitor cells could lead to the development of a better strategy for nerve injury repair.

Original language	English
Pages (from-to)	354-362
Number of pages	9
Journal	Biomaterials
Volume	30
Issue number	3
DOIs	https://doi.org/10.1016/j.biomaterials.2008.09.046
State	Published - Jan 2009

Keywords

Differentiation
Electrospinning
Embryonic stem cell
Nanofibers
Nerve repair
Poly(ε-caprolactone)

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10.1016/j.biomaterials.2008.09.046

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title = "The differentiation of embryonic stem cells seeded on electrospun nanofibers into neural lineages",

abstract = "Due to advances in stem cell biology, embryonic stem (ES) cells can be induced to differentiate into a particular mature cell lineage when cultured as embryoid bodies. Although transplantation of ES cells-derived neural progenitor cells has been demonstrated with some success for either spinal cord injury repair in small animal model, control of ES cell differentiation into complex, viable, higher ordered tissues is still challenging. Mouse ES cells have been induced to become neural progenitors by adding retinoic acid to embryoid body cultures for 4 days. In this study, we examine the use of electrospun biodegradable polymers as scaffolds not only for enhancing the differentiation of mouse ES cells into neural lineages but also for promoting and guiding the neurite outgrowth. A combination of electrospun fiber scaffolds and ES cells-derived neural progenitor cells could lead to the development of a better strategy for nerve injury repair.",

keywords = "Differentiation, Electrospinning, Embryonic stem cell, Nanofibers, Nerve repair, Poly(ε-caprolactone)",

author = "Jingwei Xie and Willerth, \{Stephanie M.\} and Xiaoran Li and Macewan, \{Matthew R.\} and Allison Rader and Sakiyama-Elbert, \{Shelly E.\} and Younan Xia",

note = "Funding Information: This work was supported by a 2006 NIH Director's Pioneer Award (3DPOD000798) and start-up funds from the Washington University in St. Louis. Our warm thanks to Professor David I. Gottlieb at Department of Anatomy and Neurobiology, Washington University, St. Louis for providing us with stem cell lines. X.L. is a visiting Ph.D. student from the School of Materials Science and Engineering, Tianjin University, Tianjin, China and was partially supported by the National Council of Scholarship. ",

year = "2009",

month = jan,

doi = "10.1016/j.biomaterials.2008.09.046",

language = "English",

volume = "30",

pages = "354--362",

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AU - Xie, Jingwei

AU - Willerth, Stephanie M.

AU - Li, Xiaoran

AU - Macewan, Matthew R.

AU - Rader, Allison

AU - Sakiyama-Elbert, Shelly E.

AU - Xia, Younan

N1 - Funding Information: This work was supported by a 2006 NIH Director's Pioneer Award (3DPOD000798) and start-up funds from the Washington University in St. Louis. Our warm thanks to Professor David I. Gottlieb at Department of Anatomy and Neurobiology, Washington University, St. Louis for providing us with stem cell lines. X.L. is a visiting Ph.D. student from the School of Materials Science and Engineering, Tianjin University, Tianjin, China and was partially supported by the National Council of Scholarship.

PY - 2009/1

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N2 - Due to advances in stem cell biology, embryonic stem (ES) cells can be induced to differentiate into a particular mature cell lineage when cultured as embryoid bodies. Although transplantation of ES cells-derived neural progenitor cells has been demonstrated with some success for either spinal cord injury repair in small animal model, control of ES cell differentiation into complex, viable, higher ordered tissues is still challenging. Mouse ES cells have been induced to become neural progenitors by adding retinoic acid to embryoid body cultures for 4 days. In this study, we examine the use of electrospun biodegradable polymers as scaffolds not only for enhancing the differentiation of mouse ES cells into neural lineages but also for promoting and guiding the neurite outgrowth. A combination of electrospun fiber scaffolds and ES cells-derived neural progenitor cells could lead to the development of a better strategy for nerve injury repair.

AB - Due to advances in stem cell biology, embryonic stem (ES) cells can be induced to differentiate into a particular mature cell lineage when cultured as embryoid bodies. Although transplantation of ES cells-derived neural progenitor cells has been demonstrated with some success for either spinal cord injury repair in small animal model, control of ES cell differentiation into complex, viable, higher ordered tissues is still challenging. Mouse ES cells have been induced to become neural progenitors by adding retinoic acid to embryoid body cultures for 4 days. In this study, we examine the use of electrospun biodegradable polymers as scaffolds not only for enhancing the differentiation of mouse ES cells into neural lineages but also for promoting and guiding the neurite outgrowth. A combination of electrospun fiber scaffolds and ES cells-derived neural progenitor cells could lead to the development of a better strategy for nerve injury repair.

KW - Differentiation

KW - Electrospinning

KW - Embryonic stem cell

KW - Nanofibers

KW - Nerve repair

KW - Poly(ε-caprolactone)

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DO - 10.1016/j.biomaterials.2008.09.046

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SN - 0142-9612

VL - 30

SP - 354

EP - 362

JO - Biomaterials

JF - Biomaterials

IS - 3

ER -

The differentiation of embryonic stem cells seeded on electrospun nanofibers into neural lineages

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