TY - JOUR
T1 - The dietary restriction-like gene drl-1, which encodes a putative serine/threonine kinase, is essential for orsay virus infection in caenorhabditis elegans
AU - Sandoval, Luis Enrique
AU - Jiang, Hongbing
AU - Wang, David
N1 - Publisher Copyright:
© 2019 American Society for Microbiology.
PY - 2019/2/1
Y1 - 2019/2/1
N2 - Orsay virus is the only known natural virus pathogen of Caenorhabditis elegans, and its discovery has enabled virus-host interaction studies in this model organism. Host genes required for viral infection remain understudied. We previously established a forward genetic screen based on a virus-inducible green fluorescent protein transcriptional reporter to identify novel host factors essential for virus infection. Here, we report the essential role in Orsay virus infection of the dietary restriction-like (drl-1) gene, which encodes a serine/threonine kinase similar to the mammalian MEKK3 kinase. Ablation of drl-1 led to a10,000-fold reduction in Orsay virus RNA levels, which could be rescued by ectopic expression of DRL-1. DRL-1 was dispensable for Orsay replication from an endogenous transgene replicon, suggesting that DRL-1 affects a prereplication stage of the Orsay life cycle. Thus, this study demonstrates the power of C. elegans as a model to identify novel virus-host interactions essential for virus infection.
AB - Orsay virus is the only known natural virus pathogen of Caenorhabditis elegans, and its discovery has enabled virus-host interaction studies in this model organism. Host genes required for viral infection remain understudied. We previously established a forward genetic screen based on a virus-inducible green fluorescent protein transcriptional reporter to identify novel host factors essential for virus infection. Here, we report the essential role in Orsay virus infection of the dietary restriction-like (drl-1) gene, which encodes a serine/threonine kinase similar to the mammalian MEKK3 kinase. Ablation of drl-1 led to a10,000-fold reduction in Orsay virus RNA levels, which could be rescued by ectopic expression of DRL-1. DRL-1 was dispensable for Orsay replication from an endogenous transgene replicon, suggesting that DRL-1 affects a prereplication stage of the Orsay life cycle. Thus, this study demonstrates the power of C. elegans as a model to identify novel virus-host interactions essential for virus infection.
KW - Caenorhabditis elegans
KW - Drl-1
KW - Orsay virus
UR - http://www.scopus.com/inward/record.url?scp=85060163770&partnerID=8YFLogxK
U2 - 10.1128/JVI.01400-18
DO - 10.1128/JVI.01400-18
M3 - Article
C2 - 30429346
AN - SCOPUS:85060163770
SN - 0022-538X
VL - 93
JO - Journal of virology
JF - Journal of virology
IS - 3
M1 - e01400-18
ER -