TY - JOUR
T1 - The dietary restriction-like gene drl-1, which encodes a putative serine/threonine kinase, is essential for orsay virus infection in caenorhabditis elegans
AU - Sandoval, Luis Enrique
AU - Jiang, Hongbing
AU - Wang, David
N1 - Funding Information:
We thank Michael Nonet for sharing the WRM0633dH12 fosmid. Some strains were provided by the CGC, which is funded by the NIH Office of Research Infrastructure Programs (P40 OD010440). L.E.S. is supported in part by the NSF Graduate Research Fellowship Program (grant DGE-1745038). This project was supported in part by NIH grant AI134967 to D.W. and American Heart Association grant 18TPA34230015 to D.W.
Publisher Copyright:
© 2019 American Society for Microbiology.
PY - 2019/2/1
Y1 - 2019/2/1
N2 - Orsay virus is the only known natural virus pathogen of Caenorhabditis elegans, and its discovery has enabled virus-host interaction studies in this model organism. Host genes required for viral infection remain understudied. We previously established a forward genetic screen based on a virus-inducible green fluorescent protein transcriptional reporter to identify novel host factors essential for virus infection. Here, we report the essential role in Orsay virus infection of the dietary restriction-like (drl-1) gene, which encodes a serine/threonine kinase similar to the mammalian MEKK3 kinase. Ablation of drl-1 led to a10,000-fold reduction in Orsay virus RNA levels, which could be rescued by ectopic expression of DRL-1. DRL-1 was dispensable for Orsay replication from an endogenous transgene replicon, suggesting that DRL-1 affects a prereplication stage of the Orsay life cycle. Thus, this study demonstrates the power of C. elegans as a model to identify novel virus-host interactions essential for virus infection.
AB - Orsay virus is the only known natural virus pathogen of Caenorhabditis elegans, and its discovery has enabled virus-host interaction studies in this model organism. Host genes required for viral infection remain understudied. We previously established a forward genetic screen based on a virus-inducible green fluorescent protein transcriptional reporter to identify novel host factors essential for virus infection. Here, we report the essential role in Orsay virus infection of the dietary restriction-like (drl-1) gene, which encodes a serine/threonine kinase similar to the mammalian MEKK3 kinase. Ablation of drl-1 led to a10,000-fold reduction in Orsay virus RNA levels, which could be rescued by ectopic expression of DRL-1. DRL-1 was dispensable for Orsay replication from an endogenous transgene replicon, suggesting that DRL-1 affects a prereplication stage of the Orsay life cycle. Thus, this study demonstrates the power of C. elegans as a model to identify novel virus-host interactions essential for virus infection.
KW - Caenorhabditis elegans
KW - Drl-1
KW - Orsay virus
UR - http://www.scopus.com/inward/record.url?scp=85060163770&partnerID=8YFLogxK
U2 - 10.1128/JVI.01400-18
DO - 10.1128/JVI.01400-18
M3 - Article
C2 - 30429346
AN - SCOPUS:85060163770
SN - 0022-538X
VL - 93
JO - Journal of virology
JF - Journal of virology
IS - 3
M1 - e01400-18
ER -