The delta opioid receptor tool box

Ana Vicente-Sanchez, Laura Segura, Amynah A. Pradhan

Research output: Contribution to journalReview articlepeer-review

27 Scopus citations

Abstract

In recent years, the delta opioid receptor has attracted increasing interest as a target for the treatment of chronic pain and emotional disorders. Due to their therapeutic potential, numerous tools have been developed to study the delta opioid receptor from both a molecular and a functional perspective. This review summarizes the most commonly available tools, with an emphasis on their use and limitations. Here, we describe (1) the cell-based assays used to study the delta opioid receptor. (2) The features of several delta opioid receptor ligands, including peptide and non-peptide drugs. (3) The existing approaches to detect delta opioid receptors in fixed tissue, and debates that surround these techniques. (4) Behavioral assays used to study the in vivo effects of delta opioid receptor agonists; including locomotor stimulation and convulsions that are induced by some ligands, but not others. (5) The characterization of genetically modified mice used specifically to study the delta opioid receptor. Overall, this review aims to provide a guideline for the use of these tools with the final goal of increasing our understanding of delta opioid receptor physiology.

Original languageEnglish
Pages (from-to)145-159
Number of pages15
JournalNeuroscience
Volume338
DOIs
StatePublished - Dec 3 2016

Keywords

  • G protein-coupled receptor
  • cell lines
  • mutant mice
  • pain

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