TY - JOUR
T1 - The defensin-related murine CRS1C gene
T2 - Expression in paneth cells and linkage to Defcr, the cryptdin locus
AU - Lin, Michael Y.
AU - Munshi, Imtiaz A.
AU - Ouellette, Andre J.
N1 - Funding Information:
This work was supported by Grant 15888 from the Shriners Hospitals for Crippled Children. We thank Dr. Wayne Frankel for useful discussions and Dana Frederick and Hong Yang for excellent technical assistance.
PY - 1992/10
Y1 - 1992/10
N2 - The site of defensin-related CRS1C gene expression in mouse small bowel and the chromosomal location of the CRS1C locus, Defcr-rauthor, have been determined. CRS1C (cryptdin-related sequence 1C) mRNA is an abundant small intestinal sequence that exhibits extensive similarity to the prepro-coding regions of defensin mRNAs yet does not encode a defensin (A. J. Ouellette and J. C. Lualdi, 1990, J. Biol. Chem. 265: 9831-9837). Using sequence-specific probes, CRS1C mRNA was detected in Paneth cells at the base of intestinal crypts by in situ hybridization. Southern blot analysis of genomic DNAs from inbred and recombinant inbred (RI) mouse strains, also conducted with probes specific for CRS1C, showed that the CRS1C locus maps to the proximal region of Chromosome 8. In 62 RI strains, no discordancies were found between Defr-rauthor and Defcr, the cryptdin gene. Thus, both the Defcr-rauthor and the Defcr genes are expressed in Paneth cells and both are genetically inseparable within 1.58 cM on Chromosome 8. These studies identify a second defensin-related Paneth cell gene in mice.
AB - The site of defensin-related CRS1C gene expression in mouse small bowel and the chromosomal location of the CRS1C locus, Defcr-rauthor, have been determined. CRS1C (cryptdin-related sequence 1C) mRNA is an abundant small intestinal sequence that exhibits extensive similarity to the prepro-coding regions of defensin mRNAs yet does not encode a defensin (A. J. Ouellette and J. C. Lualdi, 1990, J. Biol. Chem. 265: 9831-9837). Using sequence-specific probes, CRS1C mRNA was detected in Paneth cells at the base of intestinal crypts by in situ hybridization. Southern blot analysis of genomic DNAs from inbred and recombinant inbred (RI) mouse strains, also conducted with probes specific for CRS1C, showed that the CRS1C locus maps to the proximal region of Chromosome 8. In 62 RI strains, no discordancies were found between Defr-rauthor and Defcr, the cryptdin gene. Thus, both the Defcr-rauthor and the Defcr genes are expressed in Paneth cells and both are genetically inseparable within 1.58 cM on Chromosome 8. These studies identify a second defensin-related Paneth cell gene in mice.
UR - http://www.scopus.com/inward/record.url?scp=0026481214&partnerID=8YFLogxK
U2 - 10.1016/S0888-7543(05)80227-7
DO - 10.1016/S0888-7543(05)80227-7
M3 - Article
C2 - 1427853
AN - SCOPUS:0026481214
SN - 0888-7543
VL - 14
SP - 363
EP - 368
JO - Genomics
JF - Genomics
IS - 2
ER -