TY - JOUR
T1 - The Dark Kinase Knowledgebase
T2 - An online compendium of knowledge and experimental results of understudied kinases
AU - Berginski, Matthew E.
AU - Moret, Nienke
AU - Liu, Changchang
AU - Goldfarb, Dennis
AU - Sorger, Peter K.
AU - Gomez, Shawn M.
N1 - Funding Information:
We thank other members of the IDG-Kinase Data and Resource Generating Center, including PIs Gary Johnson (UNC), Tim Willson (UNC), Ben Major (WUSTL) and Reid Townsend (WUSTL). We also thank the UNC School of Medicine for providing hosting services for the DKK and expression browser. National Institutes of Health Illuminating the Druggable Genome Program [U24DK116204]. Funding for open access charge: NIH [U24DK116204].
Publisher Copyright:
© The Author(s) 2020. Published by Oxford University Press on behalf of Nucleic Acids Research. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.
PY - 2021/1/8
Y1 - 2021/1/8
N2 - Kinases form the backbone of numerous cell signaling pathways, with their dysfunction similarly implicated in multiple pathologies. Further facilitated by their druggability, kinases are a major focus of therapeutic development efforts in diseases such as cancer, infectious disease and autoimmune disorders. While their importance is clear, the role or biological function of nearly one-third of kinases is largely unknown. Here, we describe a data resource, the Dark Kinase Knowledgebase (DKK; https://darkkinome. org), that is specifically focused on providing data and reagents for these understudied kinases to the broader research community. Supported through NIH's Illuminating the Druggable Genome (IDG) Program, the DKK is focused on data and knowledge generation for 162 poorly studied or 'dark' kinases. Types of data provided through the DKK include parallel reaction monitoring (PRM) peptides for quantitative proteomics, protein interactions, NanoBRET reagents, and kinase-specific compounds. Higher-level data is similarly being generated and consolidated such as tissue gene expression profiles and, longer-term, functional relationships derived through perturbation studies. Associated web tools that help investigators interrogate both internal and external data are also provided through the site. As an evolving resource, the DKK seeks to continually support and enhance knowledge on these potentially high-impact druggable targets.
AB - Kinases form the backbone of numerous cell signaling pathways, with their dysfunction similarly implicated in multiple pathologies. Further facilitated by their druggability, kinases are a major focus of therapeutic development efforts in diseases such as cancer, infectious disease and autoimmune disorders. While their importance is clear, the role or biological function of nearly one-third of kinases is largely unknown. Here, we describe a data resource, the Dark Kinase Knowledgebase (DKK; https://darkkinome. org), that is specifically focused on providing data and reagents for these understudied kinases to the broader research community. Supported through NIH's Illuminating the Druggable Genome (IDG) Program, the DKK is focused on data and knowledge generation for 162 poorly studied or 'dark' kinases. Types of data provided through the DKK include parallel reaction monitoring (PRM) peptides for quantitative proteomics, protein interactions, NanoBRET reagents, and kinase-specific compounds. Higher-level data is similarly being generated and consolidated such as tissue gene expression profiles and, longer-term, functional relationships derived through perturbation studies. Associated web tools that help investigators interrogate both internal and external data are also provided through the site. As an evolving resource, the DKK seeks to continually support and enhance knowledge on these potentially high-impact druggable targets.
UR - http://www.scopus.com/inward/record.url?scp=85099427996&partnerID=8YFLogxK
U2 - 10.1093/nar/gkaa853
DO - 10.1093/nar/gkaa853
M3 - Article
C2 - 33079988
AN - SCOPUS:85099427996
SN - 0305-1048
VL - 49
SP - D529-D535
JO - Nucleic Acids Research
JF - Nucleic Acids Research
IS - D1
ER -