TY - JOUR
T1 - The cysteine regulatory complex from plants and microbes
T2 - What was old is new again
AU - Jez, Joseph M.
AU - Dey, Sanghamitra
PY - 2013/4
Y1 - 2013/4
N2 - The physical organization of enzymes in metabolism is an old concept being revisited by new experimental approaches. In plants and microbes, the enzymes of cysteine biosynthesis serine acetyltransferase (SAT) and O-acetylserine sulfhydrylase (OASS) form a bi-enzyme complex called the cysteine regulatory complex (CRC), which likely plays a role in modulating cysteine biosynthesis in response to sulfur nutrient state. Structural and biochemical studies of SAT and OASS as individual enzymes and recent advances in structural, biophysical, and in vivo analysis of the CRC provide new insights on the function of this macromolecular assembly in plants and microbes and opens biotechnology and pharmaceutical opportunities for future exploration.
AB - The physical organization of enzymes in metabolism is an old concept being revisited by new experimental approaches. In plants and microbes, the enzymes of cysteine biosynthesis serine acetyltransferase (SAT) and O-acetylserine sulfhydrylase (OASS) form a bi-enzyme complex called the cysteine regulatory complex (CRC), which likely plays a role in modulating cysteine biosynthesis in response to sulfur nutrient state. Structural and biochemical studies of SAT and OASS as individual enzymes and recent advances in structural, biophysical, and in vivo analysis of the CRC provide new insights on the function of this macromolecular assembly in plants and microbes and opens biotechnology and pharmaceutical opportunities for future exploration.
UR - http://www.scopus.com/inward/record.url?scp=84878606886&partnerID=8YFLogxK
U2 - 10.1016/j.sbi.2013.02.011
DO - 10.1016/j.sbi.2013.02.011
M3 - Review article
C2 - 23510784
AN - SCOPUS:84878606886
SN - 0959-440X
VL - 23
SP - 302
EP - 310
JO - Current Opinion in Structural Biology
JF - Current Opinion in Structural Biology
IS - 2
ER -