TY - JOUR
T1 - The current state of animal models and genomic approaches towards identifying and validating molecular determinants of Mycobacterium tuberculosis infection and tuberculosis disease
AU - Bucsan, Allison N.
AU - Mehra, Smriti
AU - Khader, Shabaana A.
AU - Kaushal, Deepak
N1 - Publisher Copyright:
© 2019 FEMS 2019.
PY - 2019/6/1
Y1 - 2019/6/1
N2 - Animal models are important in understanding both the pathogenesis of and immunity to tuberculosis (TB). Unfortunately, we are beginning to understand that no animal model perfectly recapitulates the human TB syndrome, which encompasses numerous different stages. Furthermore, Mycobacterium tuberculosis infection is a very heterogeneous event at both the levels of pathogenesis and immunity. This review seeks to establish the current understanding of TB pathogenesis and immunity, as validated in the animal models of TB in active use today. We especially focus on the use of modern genomic approaches in these models to determine the mechanism and the role of specific molecular pathways. Animal models have significantly enhanced our understanding of TB. Incorporation of contemporary technologies such as single cell transcriptomics, high-parameter flow cytometric immune profiling, proteomics, proteomic flow cytometry and immunocytometry into the animal models in use will further enhance our understanding of TB and facilitate the development of treatment and vaccination strategies.
AB - Animal models are important in understanding both the pathogenesis of and immunity to tuberculosis (TB). Unfortunately, we are beginning to understand that no animal model perfectly recapitulates the human TB syndrome, which encompasses numerous different stages. Furthermore, Mycobacterium tuberculosis infection is a very heterogeneous event at both the levels of pathogenesis and immunity. This review seeks to establish the current understanding of TB pathogenesis and immunity, as validated in the animal models of TB in active use today. We especially focus on the use of modern genomic approaches in these models to determine the mechanism and the role of specific molecular pathways. Animal models have significantly enhanced our understanding of TB. Incorporation of contemporary technologies such as single cell transcriptomics, high-parameter flow cytometric immune profiling, proteomics, proteomic flow cytometry and immunocytometry into the animal models in use will further enhance our understanding of TB and facilitate the development of treatment and vaccination strategies.
KW - Mycobacterium tuberculosis
KW - animal models
KW - translation
UR - http://www.scopus.com/inward/record.url?scp=85071350165&partnerID=8YFLogxK
U2 - 10.1093/femspd/ftz037
DO - 10.1093/femspd/ftz037
M3 - Review article
C2 - 31381766
AN - SCOPUS:85071350165
SN - 2049-632X
VL - 77
JO - Pathogens and disease
JF - Pathogens and disease
IS - 4
M1 - ftz037
ER -