The cortical protein Num1p is essential for dynein-dependent interactions of microtubules with the cortex

Richard A. Heil-Chapdelaine, Jessica R. Oberle, John A. Cooper

Research output: Contribution to journalArticlepeer-review

125 Scopus citations

Abstract

In budding yeast, the mitotic spindle moves into the neck between the mother and bud via dyneindependent sliding of cytoplasmic microtubules along the cortex of the bud. How dynein and microtubules interact with the cortex is unknown. We found that cells lacking Num1p failed to exhibit dynein-dependent microtubule sliding in the bud, resulting in defective mitotic spindle movement and nuclear segregation. Num1p localized to the bud cortex, and that localization was independent of microtubules, dynein, or dynactin. These data are consistent with Num1p being an essential element of the cortical attachment mechanism for dynein-dependent sliding of microtubules in the bud.

Original languageEnglish
Pages (from-to)1337-1343
Number of pages7
JournalJournal of Cell Biology
Volume151
Issue number6
DOIs
StatePublished - Dec 11 2000

Keywords

  • Dynein
  • Microtubule attachment
  • Mitosis
  • Num1p
  • Yeast

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